哺乳动物单细胞研究技术的现状与未来  

Methods for mammalian single cell research-a review

作　　者：姜文倩 田亚茸 左锐 林峻[1,4,2] Wenqian Jiang;Yarong Tian;Rui Zuo;Jun Lin(Institute of Applied Genomics, Fuzhou University, Fuzhou 350108, Fujian, China;Department of Infectious Diseases, Institute of Biomedicine, University of Gothenburg, Gothenburg 40530, Sweden;School of Basic Medical Sciences, Fujian Medical University, Fuzhou 350122, Fujian, China;College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, Fujian, China)

机构地区：[1]福州大学应用基因组学研究所,福建福州350108 [2]福州大学生物科学与工程学院,福建福州350108 [3]哥德堡大学生物医药研究所感染性疾病中心,瑞典哥德堡40530 [4]福建医科大学基础医学院,福建福州350122

出　　处：《生物工程学报》2019年第1期27-39,共13页Chinese Journal of Biotechnology

基　　金：国家自然科学基金(Nos.31301537;31371287;31571300);福建省中青年教师教育科研项目(No.JAT170080)资助~~

摘　　要：近年来,生命科学和医学的基础研究已深入到单细胞阶段。单细胞研究为揭示生命活动的基本规律、探索细胞异质性、提高对疾病发病机制的认识等提供了重要的线索和依据,同时,单细胞技术已被应用于日常实践中,如法医学和临床生殖医学。单细胞研究中使用的技术也在不断变化,并越来越复杂。文中主要介绍单细胞分离技术,包括手工挑取、激光捕获显微切割和微流控技术,以及单细胞中DNA、RNA和蛋白质分析方法的各种技术。此外,文中总结了近年来生命科学和医学领域的主要单细胞研究成果,讨论了单细胞相关技术和研究的不足,并介绍了其未来的发展方向。Basic research in life science and medicine has dug into single cell level in recent years.Single-cell analysis offers to understand life from diverse perspectives and is used to profile cell heterogeneity to investigate mechanism of diseases.Single cell technologies have also found applications in forensic medicine and clinical reproductive medicine,while the techniques are rapidly evolving and have become more and more sophisticated.In this article,we reviewed various single cell isolation techniques and their pros and cons,including manual cell picking,laser capture microdissection andmicrofluidics,as well as analysis methods for DNA,RNA and protein in single cell.In addition,we summarized major up-to-date single cell research achievements and their potential applications.

关 键 词：单细胞 激光捕获显微切割 微流控 单细胞基因组测序 单细胞转录组测序 

分 类 号：Q813[生物学—生物工程]