三七总皂苷对人胃癌SGC-7901细胞增殖、侵袭及凋亡的影响  

Effect of Panax notoginseng on proliferation,invasion and apoptosis of gastric cancer SGC-7901 cells

作　　者：高晓会[1] 张亚利[1] 张治业[1] 郭双双[1] 郭艳珍[1] GAO Xiaohui;ZHANG Yali;ZHANG Zhiye;GUO Shuangshuang;GUO Yanzhen(Department of Oncology,the First Affiliated Hospital of He′nan University of Science and Technology,Luoyang 471003,China)

机构地区：[1]河南科技大学第一附属医院肿瘤内科,洛阳471003

出　　处：《西北药学杂志》2019年第2期245-250,共6页Northwest Pharmaceutical Journal

基　　金：河南省卫生科技创新型人才工程优秀人员项目(编号:cxyx20130407)

摘　　要：目的探讨三七总皂苷(Panax notoginseng,PNS)治疗胃癌(gastric cancer,GC)的潜在分子机制,为临床PNS治疗GC奠定理论基础。方法将培养的人胃癌SGC-7901细胞随机分为4组:对照组(C组,只加入生理盐水),低质量浓度组(L组,PNS:200μg·mL^(-1)),中质量浓度组(M组,PNS:50μg·mL^(-1))和高质量浓度组(H组,PNS:100μg·mL^(-1))。PNS给药24,48,72和96 h后,采用CCK-8法检测人胃癌SGC-7901细胞活力。使用流式细胞仪检测PNS对胃癌SGC-7901细胞周期的影响。在给PNS 48 h后,通过Hoechst-33342荧光染色探索胃癌SGC-7901细胞凋亡情况。通过Transwell试验,检测不同质量浓度PNS对SGC-7901细胞侵袭的影响。同时,利用蛋白免疫印迹实验检测PNS对胃癌SGC-7901细胞Bax、Bcl-2蛋白表达的影响。结果 PNS能够抑制体外人胃癌SGC-7901细胞的增殖活力。给药24 h后,H组细胞活力明显低于C组(P<0.05);给药48和72 h后,3个实验组细胞活力均明显低于C组(P<0.05);给药48和72 h后,H组细胞活力均明显低于L组和M组;给药72 h后,M组细胞活力明显低于L组(P<0.05)。流式细胞仪结果显示,与C组比较,3个组G0/G1期细胞数量均明显增加,而S期细胞数量均明显减少(P<0.05);与L组比较,H组G0/G1期细胞数量均明显增加,而S期细胞数量均明显减少(P<0.05)。细胞凋亡实验结果表明,与C组比较,3个组细胞凋亡率均明显提高(P<0.05);与L组比较,M组、H组细胞凋亡率明显提高(P<0.05);与M组比较,H组细胞凋亡率明显提高(P<0.05)。Transwell侵袭实验结果显示,PNS能以质量浓度依赖方式抑制胃癌SGC-7901细胞的侵袭。与C组比较,3个组细胞穿膜数量明显减少(P<0.05);与L组和M组比较,H组细胞穿膜数量明显减少(P<0.05)。蛋白免疫印迹实验结果显示,与C组比较,3个组Bax蛋白表达明显增加(P<0.05);与L组比较,M组和H组Bax蛋白表达明显增加(P<0.05);与M组比较,H组Bax蛋白表达明显增加(P<0.05)。与C组比较,3个组Bcl-2蛋白表达明显减�Objective To investigate the underlying molecular mechanism of Panax notoginseng (PNS) administration in the treatment of gastric cancer (GC),and lay a theoretical foundation for clinical GC treatment using PNS.Methods The cultured human gastric cancer SGC-7901 cells were randomly divided into 4 groups:control group (C group,only adding saline solution),low group (L group,adding PNS:50 μg·mL^-1 ),median group (M group,adding PNS:100 μg·mL^-1 ),and high group (H group,adding PNS:200 μg·mL^-1 ).CCK-8 assay was employed to evaluate the cell proliferation of SGC-7901 cells at 24,48,72 and 96 h after PNS administration.The effect of PNS administration on cell cycle was detected by using a flow cytometer.Hoechst-33342 fluorescent staining was utilized to test the apoptosis of SGC-7901 cells at 48 h after adding PNS.Meanwhile,transwell migration assay was performed to determine the effect of PNS administration on cell invasion.Western blot experiment was used to determine the protein expression of Bax and Bcl-2 after PNS administration.Results The results showed that PNS administration could inhibit the viability of SGC-7901 cells.The cell viability in H group was significantly lower than that in C group at 24 h after PNS administration ( P <0.05).The cell viability in L group,M group and H group was significantly decreased compared with C group,and which of in H group was obviously lower compared with L group and M group at 48 h and 72 h after PNS administration ( P <0.05).Meanwhile,the cell number in G0/G1 phase was significantly increased in L group,M group and H group when compared with C group ( P <0.05),but the cell number in S phase was overtly decreased indicated by flow cytometer ( P <0.05);the cell number in G0/G1 phase was significantly increased in H group compared with L group ( P <0.05),while the results were opposite when the cell cycle was S phase.The analyses of apoptosis showed that the cell apoptosis rate in L group,M group and H group was significantly increased compared with C group ( P <0.05)

关 键 词：三七总皂苷 胃癌 增殖 侵袭 

分 类 号：R975[医药卫生—药品]