雷公藤甲素对中国仓鼠卵巢细胞凋亡的影响及黄体酮的干预效果  

Apoptosis effects of triptolide on ovary cells and the intervention effect of progesterone in Chinese hamster

作　　者：刘宴娟 祁爱蓉[1] 路遥 李顺民[1] LIU Yan-juan;QI Ai-rong;LU Yao;LI Shun-min(Department of Nephrology,the Fourth Clinical Medical College of Guangzhou University of Chinese Medicine,Shenzhen 518033,China;Department of Nephrology,Shenzhen Hospital of Southern Medical University,Shenzhen 518033,China)

机构地区：[1]广州中医药大学第四临床医学院肾病科,广东省深圳市518033 [2]南方医科大学深圳医院肾内科,广东省深圳市518033

出　　处：《广西医学》2019年第3期341-343,365,共4页Guangxi Medical Journal

基　　金：广东省深圳市卫生和计划生育委员会临床技术研究及转化类科研项目(201501040);广东省中医药局中医药常规科研项目(20161225)

摘　　要：目的探讨雷公藤甲素对中国仓鼠卵巢(CHO)细胞凋亡的影响及黄体酮的干预效果。方法将CHO细胞分为对照组、雷公藤甲素组、黄体酮组、雷公藤甲素+黄体酮组,分别给予等量培养液、0. 05μg/μl雷公藤甲素、0. 05μg/μl黄体酮、0. 05μg/μl雷公藤甲素联合0. 05μg/μl黄体酮干预。分别于干预后6 h及12 h检测4组CHO细胞凋亡率、细胞外信号调节蛋白激酶(ERK)磷酸化及即早基因c-fos表达水平。结果雷公藤甲素组干预后6 h及12 h的细胞凋亡率均高于对照组和黄体酮组(均P <0. 05),但两个时间点的细胞凋亡率差异无统计学意义(P> 0. 05)。雷公藤甲素+黄体酮组干预后6 h的细胞凋亡率高于对照组和黄体酮组,而低于雷公藤甲素组(均P <0. 05),且干预后12 h的细胞凋亡率低于干预后6 h(P <0. 05)。雷公藤甲素组的ERK磷酸化水平低于对照组,c-fos水平高于对照组,雷公藤甲素+黄体酮组的ERK磷酸化水平和c-fos水平均低于对照组和雷公藤甲素组(均P <0. 05)。结论雷公藤甲素可促进CHO细胞凋亡,可能与其引起ERK/c-fos异常表达有关,但干预时间延长并不增加细胞凋亡程度。黄体酮对雷公藤甲素所致仓鼠卵巢细胞损害具有保护功能。Objective To investigate the apoptosis effects of triptolide on ovary(CHO) cells and the intervention effect of progesterone in Chinese hamster.Methods CHO cells were divided into control group,triptolide group,progesterone group,oRtriptolide+progesterone group,and treated with equivalent nutrient solution,0.05 μg/μl triptolide,0.05 μg/μl progesterone,0.05 μg/μl triptolide combine with 0.05 μg/μl progesterone,respectively.The apoptosis rate of CHO cells,phosphorylation of extracellulaRsignal-regulated protein kinase(ERK) and expression level of immediately early gene c-fos were detected afteR6 and 12 hours of intervention.Results The apoptosis rate of CHO cells in triptolide group was higheRthan that in control group oRprogesterone group afteR6 and 12 hours of intervention (all P <0.05),but no statistical differences in apoptosis rates of two time points were found( P >0.05).The apoptosis rate of CHO cells in triptolide+progesterone group was higheRthan that in control group and progesterone group,but loweRthan that in triptolide group afteR6 hours of intervention(all P <0.05),and the rate was even loweRafteR12 hours of intervention( P <0.05).The phosphorylation level of ERK was lower,and the expression level of c-fos was higheRin triptolide group than in the control,and the phosphorylation level of ERK and expression level of c-fos were loweRin the triptolide+progesterone group than in the control group oRtriptolide group(all P <0.05).Conclusion Triptolide can promote the apoptosis of CHO cells,which might be related to the abnormal expression of ERK/c-fos.However,with the prolonged intervention time of triptolide,the degree of apoptosis does not increase.Progesterone has a protective function against injure of CHO cells induced by triptolide in hamster.

关 键 词：卵巢细胞 细胞凋亡 雷公藤甲素 黄体酮 

分 类 号：R711[医药卫生—妇产科学]