华木莲叶片愈伤组织诱导研究  

Study on Callus Induction from Leaf Explants of Sinomanglietia glauca

作　　者：尤润 曹璐瑶 廖文轩 王玲玲 王文秀 钟丽萍 郑丽丽 邢梦雪 肖祖飞[1] You Run;Cao Luyao;Liao Wenxuan;Wang Lingling;Wang Wenxiu;Zhong Liping;Zheng Lili;Xing Mengxue;Xiao Zufei(School of Hydraulic and Ecological Engineering, Nanchang institute technology,Jiangxi,330099)

机构地区：[1]南昌工程学院水利与生态工程学院,南昌330099

出　　处：《湖北林业科技》2019年第1期11-14,75,共5页Hubei Forestry Science and Technology

基　　金：江西省教育厅科技项目(GJJ151114);2015年地方高校国家级大学生创新创业训练计划资助项目(201511319001);2017年地方高校国家级大学生创新创业训练计划资助项目(201711319015);国家自然科学基金青年科学基金(31600497)

摘　　要：以华木莲叶为材料,研究消毒时间、激素浓度配比对华木莲叶片和叶柄诱导愈伤组织的影响。结果表明:华木莲叶片和叶柄用浓度为0.1%HgCl_2消毒,消毒时间分别为8、5 min,存活率最高,分别为32.15%、49.06%。华木莲叶片诱导愈伤组织适宜激素浓度配比为1.5 mg/L6-BA+0.15 mg/LIBA,叶柄诱导愈伤组织适宜激素浓度配比为2.0 mg/L 6-BA+0.15～0.20 mg/L IBA,华木莲愈伤组织继代培养适宜培养基为MS+2.0 mg/L 6-BA+0.15 mg/L IBA。The young leaves and petioles of Sinomanglietia glauca were used as the explants in the study. The experiment studied disinfection time and hormone concentration ratio to young leaves and petioles callus induction of Sinomanglietia glauca . The results showed that the best way to disinfect leaves and petioles of Sinomanglietia glauca were using 0.1% HgCl 2 for 8min and 5min, with high survival rate and up to 32.15 % and 49.06 % respectively. The best hormone concentration ratio for leaves of Sinomanglietia glauca was 1.5 mg/L6-BA + 0.15 mg/LIBA. The best hormone concentration ratio for petioles of Sinomanglietia glauca was 2.0 mg/L6-BA + 0.15~0.20 mg/LIBA. The suitable medium for the subculture of Sinomanglietia glauca callus was MS +2.0 mg/L 6-BA +0.15 mg/LIBA.

关 键 词：华木莲 叶片 激素 愈伤组织 

分 类 号：S725.7[农业科学—林木遗传育种]