河南不同地区棉蚜种群共生菌Wolbachia的检测及系统发育分析  

Detection and phylogenetic analysis of Wolbachia in Aphis gossypii (Hemiptera: Aphididae) populations from different areas of Henan province, central China

作　　者：李绍建[1] 高蒙[1] 王娜[1] 崔小伟[1] 桑素玲[1] 范腕腕 王振宇[1] LI Shao-Jian;GAO Meng;WANG Na;CUI Xiao-Wei;SANG Su-Ling;FAN Wan-Wan;WANG Zhen-Yu(Henan Key Laboratory of Crop Pest Control, Key Laboratory of Integrated Pest Management on Crops in Southern Region of North China, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China)

机构地区：[1]河南省农业科学院植物保护研究所农业部华北南部作物有害生物综合治理重点实验室河南省农作物病虫害综合防治重点实验室,郑州450002

出　　处：《昆虫学报》2019年第2期215-221,共7页Acta Entomologica Sinica

基　　金：国家自然科学基金-河南人才培养联合基金项目(U1504317)

摘　　要：【目的】本研究旨在揭示河南省不同地区棉蚜Aphis gossypii种群共生菌Wolbachia的感染情况,明确Wolbachia的感染类型及分类地位。【方法】2016-2017年间采集河南省不同地区的13个棉蚜种群,通过扩增COI基因片段对其进行种类鉴定;通过扩增棉蚜种群中Wolbachia的wsp基因片段对其进行Wolbachia感染率的检测,应用neighbor-joining法构建系统进化树进行棉蚜种群中Wolbachia的系统发育分析。【结果】对河南省内不同地区采集的13个棉蚜种群的Wolbachia感染率而言,郑州(ZZ)种群最高(46.67%),信阳2(XY2)种群最低(6.67%),13个种群Wolbachia的感染率范围为6.67%～46.67%,平均感染率为28.35%。基于wsp基因构建的系统发育树表明,安阳和信阳的棉蚜种群感染的Wolbachia株系属于B大组,其余地区棉蚜种群感染的Wolbachia株系属于A大组。【结论】河南省不同地区的棉蚜种群Wolbachia感染率差别较大,且不同种群感染的Wolbachia株系分别属于A大组或B大组。【Aim】 This study aims to investigate the infection of secondary endosymbiont Wolbachia in Aphis gossypii populations from different areas of Henan province, central China, and further to ascertain its infection type and phylogeny.【Methods】 A. gossypii samples were collected from 13 areas of Henan province during 2016-2017, and identified by amplifying the COI gene. The infection rate of Wolbachia was detected by amplification of the wsp gene sequence from the Wolbachia in different A. gossypii populations. The phylogenetic relationship of Wolbachia in different A. gossypii populations was constructed with neighbor-joining method.【Results】 The infection rates of Wolbachia in 13 populations of A. gossypii collected from different areas of Henan province were different, being the highest (46.67%) in Zhengzhou (ZZ) population and the lowest (6.67%) in Xinyang 2 (XY2) population. The infection rates of Wolbachia in the 13 populations ranged from 6.67% to 46.67%, with the average value of 28.35%. The phylogenetic relationship based on wsp gene indicated that Wolbachia strains in both Anyang and Xinyang populations of A. gossypii were identified as supergroup B, while those in other populations were supergroup A.【Conclusion】 The infection rate of Wolbachia in different A. gossypii populations of Henan province varies distinctly, and either supergroup A or B Wolbachia is present in these A. gossypii populations.

关 键 词：棉蚜 WOLBACHIA WSP 基因 系统发育分析 感染率 株系 

分 类 号：Q965.8[生物学—昆虫学]