蛋白质组学的研究现状及其在2型糖尿病中的应用  

Application and progress of proteomics in type 2 diabetes

作　　者：张智慧 闫朝丽[1] Zhi-hui Zhang;Zhao-li Yan(Department of Endocrinology, the Affiliated Hospital of Inner Mongolia Medical University, Hohhot, Neimenggu 010059, China)

机构地区：[1]内蒙古医科大学附属医院内分泌科,内蒙古呼和浩特010059

出　　处：《中国现代医学杂志》2019年第6期31-34,共4页China Journal of Modern Medicine

基　　金：中华医学会基金(No:13040710456);内蒙古自治区自然科学基金(No:2015MS08105);内蒙古医科大学附属医院重大科研项目(No:NYFY ZD 001)

摘　　要：蛋白质组学是对基因编码蛋白质进行大规模分析的一门新兴学科。本文概述蛋白质组学的基本技术:双相凝胶电泳、多维液相色谱、生物质谱技术及蛋白质芯片在2型糖尿病(T2DM)中的应用,从而探讨T2DM中与炎症及脂代谢紊乱相关的生物蛋白:C反应蛋白、α2-巨球蛋白、载脂蛋白A1、Clusterin及锌-α-2糖蛋白的研究进展,发现早期诊断T2DM新的特异性标志物及药物作用靶点。Proteomics is a new subject for large-scale analysis of gene coding protein. This paper describes the basic technology of proteomics: application two-dimensional gel electrophoresis, multidimensional liquid chromatography, mass spectrometry, protein chip and their application in type 2 diabetes, to explore the biological protein related to inflammation and dyslipidemia in type 2 diabetes, such as C reaction protein, alpha macroglobulin, apolipoprotein 2-A1, Clusterin protein and zinc alpha-2 glycoprotein, and to find some new mellitus specific markers and drug targets for early diagnosis of type 2 diabetes.

关 键 词：蛋白质组学 糖尿病 2型 电泳 凝胶 双向 蛋白质阵列分析 

分 类 号：R587.1[医药卫生—内分泌]