不同生态环境下水稻穗部性状QTL鉴定  

Identification of QTL for Panicle Traits under Multiple Environments in Rice(Oryza sativa L.)

作　　者：刘进 姚晓云[1,2] 刘丹 余丽琴[1] 李慧[1] 王棋[2] 王嘉宇[2] 黎毛毛[1] LIU Jin;YAO Xiaoyun;LIU Dan;YU Liqin;LI Hui;WANG Qi;WANG Jiayu;LI Maomao(Rice Research Institute,Jiangxi Academy of Agricultural Sciences/National Engineering Laboratory for Rice(Nanchang)/National Rice Improvement Center(Nanchang Sub-center),Nanchang 330200,China;Rice Research Institute,Shenyang Agricultural University,Shenyang Liaoning 110866,China;Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences,Mudanjiang 157041,China)

机构地区：[1]江西省农业科学院水稻研究所/水稻国家工程实验室(南昌)/国家水稻改良中心南昌分中心,南昌330200 [2]沈阳农业大学水稻研究所,沈阳110866 [3]黑龙江省农业科学院牡丹江分院,黑龙江牡丹江157041

出　　处：《中国水稻科学》2019年第2期124-134,共11页Chinese Journal of Rice Science

基　　金：国家重点研发计划资助项目(2016YFD0100101-11);江西省青年科学基金资助项目(20171BAB214026);江西现代农业科研协同创新专项(JXXTCX201701-01);江西省农业科学院博士启动基金资助项目(20162CBS007)

摘　　要：【目的】发掘与产量相关的穗粒性状QTL对进一步克隆和利用高产基因具有重要意义。【方法】以超级粳稻龙稻5号和典型高产籼稻中优早8号杂交衍生的重组自交系群体为试材,在4种环境下对穗部性状进行比较和QTL分析。【结果】共检测到63个穗部性状QTL,分布于除第9染色体外的11条染色体上。在4个环境下分别检测到27、27、18和35个QTL。其中,16个QTL能在2个环境下被检测到,12个在3个以上环境下稳定表达,分别占QTL总数的25.40%和19.05%;第1、3、4和5染色体的多效QTL簇能在不同环境下稳定表达,对穗部性状具有明显的调控作用。【结论】第3染色体STS3.3-STS3.6区间的qSNP3、第4染色体RM5688-RM1359区间的qSNP4.1是2个新的稳定表达的多效性QTL簇。此外,上位性效应是调控穗部性状的重要组分。【Objective】It is important to explore new panicle and grain traits QTL related to yield for further cloning and utilization of high yield genes. 【Method】A recombinant inbred line(RIL) population derived from the cross between Longdao 5(super high-yielding japonica) and Zhongyouzao 8(typical indica) was used to identify QTL for panicle traits in multiple environments.【Result】Sixty-three QTLs were detected on chromosomes 1, 2, 3, 4, 5, 6, 7, 8, 10 and 12.Among them, 27, 27, 18 and 35 QTLs were detected in four ecological environments, respectively. Among these QTLs,16 QTLs were detected in two environments, 12 QTLs were detected in more than three environments, namely 25.40%and 19.05% were stably and reliably detected across multi-environments. The QTLs with pleiotropic genetic overlap effect clustered on chromosomes 1, 3, 4 and 5 were detected in multi-environments, indicating they have significant effects on panicle traits. 【Conclusion】 qSNP3(STS3.3-STS3.6) on chromosome 3 and qSNP4.1(RM5688-RM1359)on chromosome 4 may be two novel pleiotropic QTL clusters controlling panicle traits. In addition, epistatic effect plays an important role in the regulation of panicle traits.

关 键 词：水稻 生态环境 穗部性状 上位性效应 

分 类 号：Q343.15[生物学—遗传学] S511.01[农业科学—作物学]