基于透析袋模式的药物释放行为研究  

Study on release behavior of drug based on the dialysis bag

作　　者：胡平静 朱振铎 李祥子[1,2] HU Pingjing;ZHU Zhenduo;LI Xiangzi(Anhui Province Key Laboratory of Active Biological Macromolecules,Institute of Synthetic Applications of Medical Materials,Wannan Medical College,Wuhu 241002,China;School of Material Science&Engineering,Jiangsu University)

机构地区：[1]皖南医学院医用材料合成应用研究所生物活性大分子研究安徽省重点实验室,安徽芜湖241002 [2]江苏大学材料科学与工程学院

出　　处：《包头医学院学报》2019年第1期53-55,58,共4页Journal of Baotou Medical College

基　　金：皖南医学院大学生科研资助项目(WK2018S56);安徽省大学生创新创业计划(201610368119);中国博士后科学基金面上项目(2015M581731);江苏省博士后科研资助计划项目(1501066C);安徽省高校优秀青年人才支持计划重点项目(gxyqZD2016169);安徽省高校自然科学基金重点项目(KJ2015A158)

摘　　要：目的:探索阿霉素(DOX)在透析袋中释放的影响因素,获得DOX的最佳缓释条件。方法:以DOX为药物模型,通过改变透析袋的规格、缓冲介质的pH值及缓冲介质的浓度,研究不同条件下DOX的累积缓释率和缓释平衡时间,利用扫描电子显微镜(SEM)对透析袋的显微结构进行表征,利用紫外-可见分光光度计(UV-Vis)检测释放到介质中的DOX吸光度。结果:1 000 kDa透析袋的实际平均孔径约为120 nm,3 500 Da透析袋的实际孔径<10 nm;3 500 Da透析袋中,DOX在180 min可达到释放平衡;在pH 2.0、pH 5.0及pH 7.4时,0.001、0.01、0.025 mol/L PBS介质对应的DOX累积释放率分别约为70%(pH 2.0)、86%(pH 2.0)、98%(pH 2.0)、67%(pH 5.0)、76%(pH 5.0)、94%(pH 5.0)、42%(pH 7.4)、44%(pH 7.4)、75%(pH 7.4);1 000 kDa透析袋中,DOX在160 min以内可到释放平衡;0.001、0.010、0.025 mol/L PBS介质对应的DOX累积释放率分别约为88%(pH 2.0)、93%(pH 2.0)、100%(pH 2.0)、84%(pH 5.0)、88%(pH 5.0)、98%(pH 5.0)、32%(pH 7.4)、38%(pH 7.4)、81%(pH 7.4)。结论:一定范围内,透析袋孔径越大、缓冲介质的pH值越小、缓冲介质的浓度越大,越有利于DOX的释放。本成果对透析袋模式研究纳米药物载体的体外释药性能具有重要的理论研究及实际借鉴意义。Objective:To explore the influencing factors of doxorubicin(DOX)release in dialysis bags,and to obtain the optimum release conditions.Methods:Using DOX as a drug model,the cumulative release rate and equilibration time of DOX under the different conditions were researched by changing the specifications of the dialysis bag,pH and concentration of buffer medium.The microstructures of the dialysis bags were characterized by scanning electron microscopy(SEM)and the absorbances of DOX in the medium were detected by UV-visible spectrophotometer(UV-vis).Results:The actual average pore diameter of the 1000 kDa dialysis bag was about 120 nm,but that of 3500 Da dialysis bag was less than 10 nm.Moreover,for 3500 Da dialysis bag,DOX can reach release balance in 180 min.Under three pH values of 2.0,5.0 and 7.4,the corresponding cumulative release rates of DOX in PBS medium with concentration of 0.001,0.01 and 0.025 mol/L were about 70%(pH 2.0)、86%(pH 2.0)、98%(pH 2.0)、67%(pH 5.0)、76%(pH 5.0)、94%(pH 5.0)、42%(pH 7.4)、44%(pH 7.4)、75%(pH 7.4).However,for 1000 kDa dialysis bag,DOX can reach release balance in 160 min.Under the same three pH values,the cumulative release rates of DOX in the PBS medium with concentration of 0.001,0.010 and 0.025 mol/L were about 88%(pH 2.0)、93%(pH 2.0)、100%(pH 2.0)、84%(pH 5.0)、88%(pH 5.0)、98%(pH 5.0)、32%(pH 7.4)、38%(pH 7.4)、81%(pH 7.4).Conclusion:Within a certain range,some factors such as big pore diameter of dialysis bag,low pH and high concentration of buffer medium,are beneficial to DOX-Release.The results of this study have important theoretical and practical significance for the study of in vitro drug release performance of nano-drug carrier based on dialysis bag model.

关 键 词：膜 纳米结构 优化 透析袋 阿霉素 

分 类 号：R943[医药卫生—药剂学]