基于淀粉合成基因TRAP标记的甘薯种质资源遗传多样性分析  

Genetic Diversity Analysis of Sweet Potato Germplasm Resources Based on TRAP Markers of Starch Synthesis Gene

作　　者：张道微 殷勤 董芳 黄艳岚[1] 周虹[1] 张亚[1] 张超凡[1] ZHANG Dao-wei;YIN Qin;DONG Fang;HUANG Yan-lan;ZHOU Hong;ZHANG Ya;ZHANG Chao-fan(Hunan Crop Research Institute,Changsha 410125,PRC;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,PRC)

机构地区：[1]湖南省作物研究所,湖南长沙410125 [2]湖南农业大学生物科学技术学院,湖南长沙410128

出　　处：《湖南农业科学》2018年第12期13-17,共5页Hunan Agricultural Sciences

基　　金：湖南省甘薯工程技术研究中心项目(2018TP2032);长沙市甘薯工程技术研究中心项目(kq1701024);湖南农业科技创新资金(2017XC13)

摘　　要：为探究淀粉合成相关基因在甘薯种质资源中的遗传多样性,通过对锚定引物的筛选构建了基于淀粉合成基因的TRAP分子标记技术,并对59份不同作用类型的甘薯种质资源进行遗传多样性分析。TRAP分子标记筛选结果表明:SAI/me2、AGPase/em4和β-Amylase/me2这3对引物组合在分子标记中存在较为丰富的多态性片段,结果重复性好,可作为TRAP分子标记;其中,AGPase/em4分子标记的区分能力最强,能将59份甘薯资源进行较好的区分。甘薯种质资源遗传多样性分析结果表明:不同类型的甘薯种质资源之间无明显的遗传群体区分。研究表明,甘薯基因组为六倍体起源,其淀粉合成相关基因存在多基因遗传作用,在不同甘薯种质资源之间存在一定的遗传差异,因而可以开发成有效的分子标记。To explore the genetic diversity of starch synthesis genes in sweet potato,the TRAP molecular marker based on starch synthesis genes was constructed by screening anchored primers,and genetic diversity of 59 different types of sweet potato germplasm resources was analyzed.The results show that for the 3 pairs of primer combinations SAI/me2,AGPase/em4 and beta-Amylase/me2,the polymorphic fragments are abundant and stable,and thus can be used as ideal TRAP molecular markers.Among them,AGPase/em4 molecular markers has the highest discrimination and reliability,which can distinguish 59 sweet potato resources in this test.No obvious genetic population differentiation among different types of sweet potato germplasm resources was found by the genetic diversity analysis.Previous studies have shown that the genome of sweet potato is the origin of hexaploid,and its starch synthesis genes suggests polygenic inheritance and indicates genetic diversity among different sweet potato germplasm resources.Thus the starch synthesis genes can be developed as effective molecular markers.

关 键 词：甘薯 淀粉合成基因 遗传多样性 TRAP分子标记 聚类分析 

分 类 号：S188[农业科学—农业基础科学]