1型糖尿病患者血清髓源性免疫抑制细胞与免疫球蛋白G4的意义及关系  

Relationship between myeloid-derived suppressor cells and immunoglobulin G4 in patients with type 1 diabetes mellitus

作　　者：朱志宏[1] 蔡钰玫 陈语 陈立曙[1] 陈式仪[1] 陈慎仁[1] Zhu Zhihong;Cai Yumei;Chen Yu;Chen Lishu;Chen Shiyi;Chen Shenren(Department of Endocrinology,Second Affiliated Hospital of Shantou University Medical School,Shantou 515041,China;Department of Endocrinology,Jieyang People's Hospital,Jieyang 522000,China)

机构地区：[1]汕头大学医学院第二附属医院内分泌科,515041 [2]揭阳市人民医院内分泌科,522000

出　　处：《中华临床医师杂志（电子版）》2018年第10期567-570,共4页Chinese Journal of Clinicians(Electronic Edition)

基　　金：汕头市科技计划项目(汕府科【2017】166号07)

摘　　要：目的探讨1型糖尿病(T1D)患者血清髓源性免疫抑制细胞(MDSCs)与免疫球蛋白G4(IgG4)的关系。方法选取汕头大学医学院第二附属医院于2015年1月至2017年1月期间收治的T1D患者30例,2型糖尿病(T2D)患者20例;另选取同一家医院2015年1月至2017年1月期间健康体检者20例作为对照组。采用酶联免疫吸附法测定血清IgG4浓度,流式细胞分析术检测外周血MDSCs表达频率。采用t检验以及Mann-Whitney U检验比较3组间血清IgG4浓度及MDSCs表达频率的差异,采用Spearman相关分析法分析血清IgG4与MDSCs的相关性。结果 T1D组血清IgG4为(0.92±0.79)g/L,T2D组血清IgG4为(0.54±0.33)g/L,对照组血清IgG4为(0.34±0.15)g/L,T1D血清IgG4浓度高于对照组,差异具有统计学意义(t=4.900,P <0.001),T1D组高于T2D组,差异具有统计学意义(t=2.830,P=0.007),T2D组高于对照组,差异具有统计学意义(t=2.466,P=0.010)。T1D组外周血MDSCs表达频率为(4.09±1.53)%,T2D组外周血MDSCs表达频率为(2.86±1.63)%,对照组外周血MDSCs的表达频率为(1.93±0.76)%。T1D组外周血MDSCs的表达频率明显高于对照组,差异有统计学意义(Z=-9.650,P <0.01)。T1D组MDSCs表达频率高于T2D组,差异有统计学意义(t=2.899,P=0.005)。血清IgG4与MDSCs呈正相关(r=0.482,P <0.05)。结论 T1D患者MDSCs与IgG4升高,且IgG4与MDSCs呈正相关,具有重要研究意义。Objective To assess the relationship between serum myeloid-derived suppressor cells(MDSCs)and immunoglobulin G4(IgG4)in patients with type 1 diabetes mellitus(T1DM).Methods Thirty patients with T1DM and 20 patients with T2DM were selected at the Second Affiliated Hospital of Shantou University Medical College from January 2015 to January 2017;another 20 healthy subjects were selected from January 2015 to January 2017 as a control group.The frequency of MDSCs in peripheral blood was detected by enzyme-linked immunosorbent assay and flow cytometry.The t-test,chi-square test,Mann-Whitney U test and spearman correlation analysis were used to compare the changes of serum IgG4 content,MDSCs frequency and the correlation between serum IgG4 and MDSCs.Results Serum IgG4 level was(0.92±0.79)g/L in the T1DM group,(0.54±0.33)g/L in the T2DM group,and(0.34±0.15)g/L in the normal control group.Compared with the control group,serum IgG4 levels in the T1DM and T2DM groups were significantly higher(t=4.9,P<0.001;t=2.466,P=0.01).Compared with the T2DM group,serum IgG4 level in the T1DM group was significantly higher(t=2.83,P=0.007).The frequency of MDSCs in the T1DM group was significantly higher than those in the control group(Z=-9.650,P<0.01)and in the T2DM group(t=2.899,P=0.005).Serum IgG4 levels in T1DM patients were positively correlated with the frequency of MDSCs(r=0.482,P<0.05).Conclusion MDSCs and IgG4 increase in patients with T1DM,and they have a positive correlation,which deserves further research.

关 键 词：1型糖尿病 髓源性免疫抑制细胞 免疫球蛋白G4 

分 类 号：R587.1[医药卫生—内分泌]