中国部分地区黑胸大蠊遗传多样性与系统发育地理结构研究  

Genetic diversity and phylogeographic structure of Periplaneta fuliginosa in parts of China

作　　者：徐永媛 李蓉[1] 陈艳艳[1] 陈善元[1] 肖蘅[1] XU Yong-yuan;LI Rong;CHEN Yan-yan;CHEN Shan-yuan;XIAO Heng(School of Life Sciences, Yunnan University, Kunming 650091, China)

机构地区：[1]云南大学生命科学学院,昆明650091

出　　处：《生物学杂志》2019年第1期65-69,共5页Journal of Biology

基　　金：云南省"百名海外高层次人才引进计划"项目(W8110305);云南大学引进人才科研启动资金(C6610201)

摘　　要：为了探讨中国部分地区黑胸大蠊(Periplaneta fuliginosa)遗传多样性与系统发育地理结构,采用PCR产物直接测序法测定了9个地理种群88个黑胸大蠊样本的线粒体COI基因长度为658 bp的部分序列片段,开展了黑胸大蠊种群间的遗传多样性、遗传分化、基因流和系统发育地理结构分析。结果显示:黑胸大蠊COI基因共检测到11个变异位点和7个单倍型(Hap1-Hap7),其中单倍型Hap3为9个地理种群所共享;总种群的单倍型多样度(Hd)和核苷酸多样度(Pi)分别为0. 327和0. 0017,种群的中性检验Tajima's D值为-1. 2743,结果不显著,说明黑胸大蠊在较近的历史上未经历群体扩张;总种群的遗传分化系数Gst为0. 1060;基因流Nm为4. 22,表明种群间存在较强的基因交流。系统发育树和单倍型网络图分析发现各地理种群的所有单倍型并没有单独聚在一起,同来自其他地理区域种群的单倍型相隔开; AMOVA分子变异分析显示,其遗传分化主要来自种群内部,种群间未发生明显的遗传分化; Mantel分析表明,遗传距离与地理距离间无显著相关性(R=0. 0038,P=0. 5670)。上述结果表明黑胸大蠊的遗传多样性水平相对较低,且种群间没有显著的系统发育地理结构。To investigate genetic diversity and phylogeographic structure of Periplaneta fuliginosa in some parts of China, this study applied PCR amplification and sequencing methods to determine a 658 bp fragment of partial COI gene sequences of 88 P . fuliginosa samples from 9 geographic populations. The genetic diversity, genetic differentiation, gene flow and phylogeographic structure were analyzed. The results showed that 11 variable sites and 7 haplotypes (Hap1-Hap7) were detected among all COI sequences of 9 P. fuliginosa geographic populations. Among 7 haplotypes, the haplotype Hap3 was shared by all the 9 populations. The total haplotype diversity ( Hd ) and nucleotide diversity ( Pi ) of all populations were 0.327 and 0.0017, respectively. The neutrality test Tajima′s D was -1.2743, indicating no sign of population expansion in recent history. The total Gst was 0.1060. The Nm was 4.22, showing evidence for extensive gene flow among populations. The phylogenetic tree and haplotype network analyses showed that haplotypes from different geographical regions did not cluster together and separate each region from other regions. The molecular variance analysis (AMOVA) demonstrated that genetic differentiation mainly occurred within populations, and there was no significant genetic differentiation among populations. Furthermore, a Mantel test detected no obvious correlation ( R =0.0038, P =0.5670) between genetic distance and geographic distance among P. fuliginosa populations. The results indicated that genetic diversity of P. fuliginosa was relatively low and there was no phylogeographic structuring among P. fuliginosa populations.

关 键 词：黑胸大蠊 COI基因 遗传多样性 系统发育地理结构 

分 类 号：Q953[生物学—动物学]