铁皮石斛提取物对溃疡性结肠炎BALB/c小鼠的治疗作用  

Therapeutic effects of Dendrobium officinale extract on ulcerative colitis model of BALB/c mice

作　　者：赵佩[1] 刘志龙[2] 高进贤[1] 石磊[1] 于静[1] 葛斌[1] 袁继勇[1] ZHAO Pei;LIU Zhi-long;GAO Jin-xian;SHI Lei;YU Jing;GE Bin;YUAN Ji-yong(Dept of Pharmacy, Gansu Province Hospital, Lanzhou 730000,China;Dept of Anesthesiology, Gansu Province Hospital, Lanzhou 730000,China)

机构地区：[1]甘肃省人民医院药剂科,甘肃兰州730000 [2]甘肃省人民医院麻醉科,甘肃兰州730000

出　　处：《中国药理学通报》2019年第2期235-240,共6页Chinese Pharmacological Bulletin

基　　金：甘肃省自然科学基金资助项目(No 1606RJZA138)

摘　　要：目的探讨铁皮石斛提取物对小鼠溃疡性结肠炎(ulcerative colitis,UC)的治疗作用及机制。方法 4%葡聚糖硫酸钠(DSS)诱导建立小鼠UC模型,观察造模及药物治疗期间小鼠体征变化,评估疾病活动指数(DAI),并对小鼠结肠组织进行镜下病理评分。采用Western blot检测小鼠结肠组织中MPO的表达;荧光实时定量PCR法检测结肠中IFN-γ、TNF-αmRNA的表达; ELISA法测定血清中IFN-γ、TNF-α的含量。结果与模型组比较,铁皮石斛提取物不同剂量组小鼠的结肠长度较长,DAI分数、组织学损伤评分降低(P<0.05或P<0.01);血清中IFN-γ、TNF-α含量降低(P<0.05);结肠组织中IFN-γmRNA的表达水平和MPO蛋白表达也明显下降(P<0.05)。结论铁皮石斛提取物对UC小鼠有治疗作用,其可能通过抑制IFN-γ、TNF-α释放,下调促炎因子IFN-γ、TNF-α、MPO的表达,阻断炎症的放大效应,达到治疗效果。Aim To explore the therapeutic effects of Dendrobium officinale extract on mice with ulcerative colitis(UC) and its mechanism. Methods The mice were treated with 4% DSS to induce the UC model. The signs or symptom changes of mice were observed and recorded. Then the scores of disease activity index(DAI) during the days of modeling and drug treatment were also evaluated, and the colon tissues were taken as samples. The samples were used for general and microscopic evaluation,the expression of MPO was detected by Western blot, and the expressions of IFN-γ and TNF-α mRNA were measured by qPCR.Meanwhile,the concentrations of serum IFN-γ, TNF-α in mice were also determined by ELISA. Results As compared with model group, the colon length of mice treated with Dendrobium officinale extract was longer. The DAI score, histological damage score were reduced( P <0.05 or P <0.01);the concentrations of IFN-γ and TNF-α inflammatory cytokines in serum decreased( P <0.05);the expressions of IFN-γ mRNA and MPO protein in the colon tissue significantly decreased compared with model group( P <0.05). Conclusions Dendrobium officinale extract could treat DSS-induced UC of BALB/c mice. The anti-UC mechanism of Dendrobium officinale extract can be attributed to its ability to reduce the release of IFN-γ and TNF-α and down-regulate the expressions of IFN-γ, TNF-α and MPO, to block the amplification effect of inflammation and to achieve its therapeutic effects.

关 键 词：铁皮石斛提取物 BALB/C小鼠 葡聚糖硫酸钠 溃疡性结肠炎 治疗作用 作用机制 

分 类 号：R332[医药卫生—人体生理学] R284.1[医药卫生—基础医学]