片仔癀对局灶性脑缺血/再灌注大鼠皮质中NMDAR1和GluR2表达的影响  

Effects of Pien-Tze-Huang on expression of NMDAR1 and GluR2 in cortex of MCAO rats

作　　者：张小琴[1] 侯丽颖 强国芳 顾嘉琪 卢双红 许文[1] 黄鸣清[1] ZHANG Xiao-qin;HOU Li-ying;QIANG Guo-fang;GU Jia-qi;LU Shuang-hong;XU Wen;HUANG Ming-qing(College of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《中国药理学通报》2019年第2期288-292,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81673561);福建省高校新世纪优秀人才支持计划项目(No闽教科[2015]54号);福建省自然科学基金资助项目(No 2017J01838)

摘　　要：目的研究片仔癀对局灶性脑缺血/再灌注大鼠皮质中NMDAR1、GluR2 mRNA及蛋白表达的影响。方法健康成年♂SD大鼠40只,随机分为假手术组、模型对照组、片仔癀给药组,用线栓法建立局灶性脑缺血/再灌注大鼠模型(MCAO)。Zea-Longa标准评分法评价大鼠神经功能损伤;TTC染色法评价脑梗死体积变化;qPCR法和Western blot法分别检测大鼠缺血侧脑组织皮质中NMDAR1、GluR2 mRNA及蛋白的表达。结果与MCAO组比较,片仔癀能明显改善大鼠的神经功能损伤,降低大鼠脑梗死体积,促进NMDAR1、GluR2 mRNA及蛋白的表达。结论片仔癀可能通过抑制局灶性脑缺血/再灌注大鼠皮质NMDAR1、GluR2 mRNA及蛋白的下调而发挥脑保护作用。Aim To investigate the effects of Pien-Tze-Huang(PZH) on mRNA and protein expression of NMDAR1 and GluR2 in cortex of MCAO rats. Methods Forty healthy adult male Sprague-Dawley rats were randomly divided into three groups: sham, MCAO, MCAO+PZH groups. The rats were subjected to focal cerebral ischemia/reperfusion with suture-occluded method. Neurological deficit testing was performed with Zea Longa scale. The volume of cerebral infarction was evaluated by TTC staining. The mRNA and protein expression of NMDAR1 and GluR2 in cortex of side cerebral ischemic tissues were determined using qPCR and Western blot analysis. Results Compared with MCAO group, PZH significantly improved the neurological deficit, decreased the volume of cerebral infarction, and up-regulated the mRNA and protein expression of NMDAR1 and GluR2. Conclusions PZH attenuates the down-regulation of mRNA and protein expression of NMDAR1 and GluR2 after focal cerebral ischemic injury in rats, which may be associated with the cerebral protective effect of PZH.

关 键 词：片仔癀 局灶性脑缺血/再灌注 皮质 NMDAR1 GLUR2 脑保护 

分 类 号：R332[医药卫生—人体生理学] R282.71[医药卫生—基础医学]