中红杨离体叶片植株再生体系建立  

Establishment of plant regeneration system from in vitro leaves of Populus × euramericana ‘zhonghong'

作　　者：孙红英[1,2,3] 辛全伟[1] 马志慧[3] 兰思仁[2,3] SUN Hongying;XUN Quanwei;MA Zhihui;LAN Siren(National Engineering Research Center of Fungus and Mycorrhizal Plants,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Forestry Post-doltoral Station,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China;Forestry College,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)

机构地区：[1]福建农林大学国家菌草工程技术研究中心,福建福州350002 [2]福建农林大学林学博士后科研流动站,福建福州350002 [3]福建农林大学林学院,福建福州350002

出　　处：《中南林业科技大学学报》2019年第4期28-31,共4页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金(31500265);福建农林大学第三批科技创新专项基金

摘　　要：以中红杨组培苗的叶片为试验材料,研究了基本培养基、植物生长调节物质浓度、外源添加物和光照条件对离体叶片不定芽再生的影响,并获得了完整的再生植株。结果表明:1)最适基本培养基为MS,最佳植物生长调节剂和外源添加物的组合为0.5 mg·L^(-1) 6-BA+0.2 mg·L^(-1) NAA+30 g·L^(-1)蔗糖;2)暗培养有利于愈伤组织诱导,光照16 h·d^(-1)有利于不定芽的诱导,不定芽形成率可达83.3%;3)将叶片再生植株转接到MS+0.2 mg·L^(-1) 6-BA+0.1 mg·L^(-1) NAA培养基中壮苗培养,在1/2 MS+0.2 mg·L^(-1) IBA+20 g·L^(-1)蔗糖+6.5 g·L^(-1)琼脂培养基诱导生根,生根率96.4%,生根苗大田移栽成活率86.8%。Taking the leaves of Populus × euramericana ‘zhonghong’ as tested materials, the factors affecting in vitro leaf regeneration, including basal medium, plant growth regulator, exogenous additives and illumination conditions, were studied, and some complete regenerated plants were obtained, and a high efficient system of in vitro leaf regeneration was established. The results show that: 1) The optimum basic medium was MS, and the combination of the optimal plant growth regulator and the exogenous additive was 0.5 mg·L^-1 6-BA + 0.2 mg·L^-1 NAA + 30 g·L^-1 sucrose;2) Dark culture was conducive to callus induction, illumination of 16 h·d^-1 was conducive to adventitious bud induction, adventitious bud formation rate could reach 83.3%;3) Leaf regenerated plants were transferred to MS + 0.2 mg·L^-1 6-BA + 0.1 mg·L^-1 NAA medium for strong seedling culture, the leaves were induced and rooted in 1/2 MS + 0.2 mg·L^-1 IBA + 20 g·L^-1 sucrose + 6.5 g·L^-1 agar medium, the rooting rate was 96.4%, and the survival rate of rooting seedlings in the big field was 86.8%.

关 键 词：中红杨 离体叶片培养 植株再生 生根成活 

分 类 号：S687.9[农业科学—观赏园艺]