双光子激发的超分子聚合物纳米颗粒生物成像研究  

Construction of Two-photon Excited Supramolecular Polymer Nanoparticles and Their Bioimaging Application

作　　者：刘晓琴 黄媛媛[1,2] 高建峰 陈玉哲[2] 佟振合[2] 吴骊珠[2] LIU Xiaoqin;HUANG Yuanyuan;GAO Jianfeng;CHEN Yuzhe;TONG Zhenhe(TUNG Chen-ho);WU Lizhu(School of Science, North University of China, Taiyuan 030051, Shanxi, P. R. China;Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, P. R.China)

机构地区：[1]中北大学理学院,山西太原030051 [2]中国科学院理化技术研究所光化学转换与功能材料重点实验室,北京100190

出　　处：《影像科学与光化学》2019年第2期136-145,共10页Imaging Science and Photochemistry

基　　金：国家自然科学基金项目(21871280;21474124);科技部(2014CB239402;2017YFA0206903);中国科学院战略性先导科技专项B类(XDB17000000)资助

摘　　要：将具有双-2-脲基-4[1H]-嘧啶酮(bisUPy)的β-二羰基氟硼类衍生物(BF_2-bisUPy)及卟啉衍生物(Por(Pt)-bisUPy)通过四重氢键作用组装成超分子聚合物,通过微乳液法制备成在水中均匀分散的超分子聚合物纳米颗粒(SPNP)。扫描电子显微镜形貌表征表明获得的纳米颗粒粒径约为60 nm。紫外-可见吸收光谱、荧光发射光谱及寿命衰减实验均证明纳米颗粒内BF_2-bisUPy与Por(Pt)-bisUPy可发生高效的能量传递。具有双光子吸收的BF_2-bisUPy作为能量供体,可通过荧光共振能量传递(FRET)增强双光子激发下Por(Pt)-bisUPy的发光。双光子激发荧光强度与激光功率测试表明所制备的超分子聚合物纳米颗粒具有强烈的双光子激发下的荧光及磷光双发射,这种纳米材料可进入细胞,具有优秀的生物相容性,并在双光子激发时表现出强烈的荧光和磷光双发射生物成像。A difluoroboron β-diketonate derivative(BF 2-bisUPy)and a porphyrin derivative (Por(Pt)-bisUPy)bearing bis-2-ureido-4[1H]-pyrimidinone (bisUPy)were assembled into a supramolecular polymer by quadruple hydrogen bonding, which afforded uniformly water-dispersed supramolecular polymer nanoparticles by microemulsion method. Scanning electron microscopy shows that the obtained nanoparticles have a particle size of about 60 nm. Ultraviolet-visible absorption spectroscopy, fluorescence emission spectroscopy and lifetime decay experiments have demonstrated the occurrence of efficient energy transfer between BF 2-bisUPy and Por(Pt)-bisUPy. BF 2-bisUPy has excellent two-photon absorption performance, which can be used as an energy donor to enhance the luminescence properties of Por(Pt)-bisUPy under two-photon excitation by fluorescence resonance energy transfer(FRET). The prepared supramolecular polymer nanoparticles have strong fluorescence and phosphorescence dual-emission under two-photon excitation. The nanomaterials can enter cells and exhibit excellent biocompatibility. It also exhibits bright bioimaging with dual-emission under two-photon excitation.

关 键 词：超分子聚合物 四重氢键 荧光共振能量传递 双光子 双发射生物成像 

分 类 号：TQ422[化学工程]