碳青霉烯类耐药的大肠埃希菌毒力因子与耐药基因相关性研究  

Correlation between genotype of drug-resistant genes and virulence factors of carbapenem-resistant Escherichia coli strains

作　　者：宋真[1] 金炎[1] 杨蕾[2] 路超[1] 白媛媛[1] 郝莹莹[1] Song Zhen;Jin Yan;Yang Lei;Lu Chao;Bai Yuanyuan;Hao Yingying(Clinical Laboratory of Shandong Provincial Hospital Affiliated to Shandong University, Ji’nan 250000, China)

机构地区：[1]山东大学附属省立医院检验医学部,济南250000 [2]山东大学附属省立医院医学工程管理办公室,济南250000

出　　处：《新医学》2019年第3期161-165,共5页Journal of New Medicine

基　　金：山东省自然科学基金(ZR2017BH112);山东省医药卫生科技发展计划(2017WS471);山东省重点研发计划(2016GSF201078);济南市科技发展计划(201821065)

摘　　要：目的分析碳青霉烯类耐药的大肠埃希菌(E.Coli)耐药基因型与所含的毒力因子的关系。方法 PCR检测E.Coli毒力因子和耐药基因;采用Fisher确切概率法对耐药基因和毒力因子的关系进行分析。结果 95.7%(22/23)菌株超广谱β-内酰胺酶(ESBL)基因阳性,43.5%(10/23)菌株检出胞外黏多糖相关基因,87.0%(20/23)菌株检出P菌毛相关基因。耐药基因CTX-M-15型比CTX-M-55型菌株携带毒力因子papGⅡ的概率低(P=0.049)。耐药基因NDM-1阳性菌株比NDM-5阳性菌株中毒力因子papGⅢ携带率高(P=0.047)。结论 ESBL阳性菌株中不同CTX-M、NDM亚型与毒力因子papGⅡ、papGⅢ具有一定的相关性,毒力因子的携带情况与E.Coli耐药情况可能有关。Objective To investigate the correlation between the genotype of drug-resistant genes and the virulence factors in carbapenem-resistant Escherichia coli(E.Coli) strains. Methods The virulence factors and drug-resistant genes were detected by PCR. The relationship between drug resistance genes and virulence factors was analyzed by Fisher’s exact test. Results A total of 95.7%(22/23) of the bacterial strains were positive for extended spectrum beta-lactamase( ESBLs) gene, 43.5%( 10/23) positive for the extracellular polysaccharide-related genes, and 87.0%( 20/23) positive for the P fimbriae-related genes. The positive rate of virulence factors papG Ⅱ in the CTX-M-15 strain was significantly lower compared with that in the CTX-M-55 strain( P = 0.049). The positive rate of virulence factor papG Ⅲ in the NDM-5 positive strain was considerably higher than that in NDM-1 positive strains (P = 0.047). Conclusions The genotypes of CTX-M and NDM among ESBLs positive strains were asssociated with virulence factors papG Ⅱ or papG Ⅲ virulence factors might be associated with the drug resistance genes in E.Coli.

关 键 词：大肠埃希菌 毒力因子 耐药基因 超广谱Β-内酰胺酶 碳青霉烯类耐药的肠杆菌科细菌 

分 类 号：R446.5[医药卫生—诊断学]