青蒿琥酯对人结肠癌细胞侵袭转移及TGF-β1/Smad4信号通路表达的影响  

Effects of Artesunate on Invasion and Metastasis of Human Colon Cancer Cells and the TGF-β1/Smad4 Signaling Pathway

作　　者：刘松[1] 齐放 赵宇[1] 陈克研 张升瑞[1] LIU Song;QI Fang;ZHAO Yu;CHEN Keyan;ZHANG Shengrui(Department of General Surgery,Affiliated Central Hospital of Shenyang Medical College,Shenyang 110024,China;Department of Laboratory AnimalScience,China Medical University,Shenyang 110122,China)

机构地区：[1]沈阳医学院附属中心医院普外科,沈阳110024 [2]中国医科大学实验动物部,沈阳110122

出　　处：《中国医科大学学报》2019年第1期34-38,共5页Journal of China Medical University

基　　金：国家自然科学基金青年科学基金(31201758)

摘　　要：目的探讨青蒿琥酯对结肠癌细胞侵袭转移的调控作用及对TGF-β1/Smad4信号通路表达的影响。方法利用CCK8法、裸鼠移植瘤模型、Transwell侵袭实验、流式细胞术检测青蒿琥酯对结肠癌细胞侵袭转移的调控作用;Western blotting和qRT-PCR法检测TGF-β1、Smad4蛋白和mRNA表达。结果青蒿琥酯抑制移植瘤生长;抑制细胞的增殖及侵袭,促进细胞凋亡,抑制TGF-β1表达,促进Smad4表达。TGF-β1抑制剂逆转青蒿琥酯的抑制作用。结论青蒿琥酯能抑制裸鼠移植瘤生长,其机制可能与TGF-β1/Smad4信号通路有关。Objective To investigate the mechanism via which artesunate regulates the invasion and metastasis of colon cancer cells and the expression of members of the TGF-β1/Smad4 signaling pathway. Methods The cell counting kit 8( CCK8 ),nude mouse xenograft model,Transwell invasion assay,and flow cytometry were used to investigate the effect of artesunate on the invasion and metastasis of colon cancer cells. Western blotting and quantitative reverse transcription-polymerase chain reaction( qRT-PCR) were used to detect the expression of TGF-β1 and Smad4 proteins and mRNA,respectively. Results Artesunate inhibited the growth of transplanted tumor,cell proliferation,and invasion and promoted apoptosis. It inhibited TGF-β1 expression and promoted Smad4 expression. TGF-β1 inhibitors reversed the inhibitory effect of artesunate. Conclusion Artesunate can inhibit the growth of xenograft tumor in nude mice and its mode of action may be related to the TGF-β1/Smad4 signaling pathway.

关 键 词：青蒿琥酯 结肠癌 侵袭转移 TGF-β1/Smad4 

分 类 号：R34[医药卫生—基础医学]