脂肪干细胞来源外泌体通过Wnt/β-catenin信号通路促进脂肪干细胞成骨分化  

Exosomes Promote Osteoblastic Differentiation of Adipose-Derived Stem Cells by Activating the Wnt/β-catenin Signaling Pathway

作　　者：崔梦莹 郭澍[1] 佟爽[1] 张华[1] 朱梦茹 陈义庆 魏松乔 CUI Mengying;GUO Shu;TONG Shuang;ZHANG Hua;ZHU Mengru;CHEN Yiqing;WEI Songqiao(Department of Plastic Surgery,The First Hospital,China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院整形外科,沈阳110001

出　　处：《中国医科大学学报》2019年第2期114-118,共5页Journal of China Medical University

基　　金：国家自然科学基金(51272286);国家卫生和计划生育委员会公益性科研专项(2015SQ00049);辽宁省自然科学基金(20170541040)

摘　　要：目的探讨脂肪干细胞来源的外泌体促进脂肪干细胞成骨分化过程与Wnt/β-catenin信号通路的关系。方法提取人脂肪干细胞,行成骨诱导后提取外泌体,按实验目的分组处理后,检测成骨标记物碱性磷酸酶活性,茜素红检测矿化程度,Westernblotting检测各组β-catenin蛋白表达情况。结果脂肪干细胞来源的外泌体能够促进ADSCs成骨分化,且上调β-catenin蛋白表达,当加入该通路抑制剂DKK1后,能够抑制β-catenin蛋白表达,减弱脂肪干细胞成骨分化能力。结论脂肪干细胞来源外泌体能够通过Wnt/β-catenin信号通路促进脂肪干细胞成骨分化。Objective To investigate the relationship between promotion of osteogenic differentiation of adipose-derived stem cells(ADSCs)by exosomes secreted by ADSCs and the Wnt/β-catenin signaling pathway.Methods ADSCs were extracted in the Plastic Surgery Department of the First Hospital Affiliated to China Medical University.Exosomes were extracted from ADSCs induced to differentiate into osteoblasts.The ADSCs were separated into four groups and used for measuring activity of alkaline phosphatase(ALP)by using enzyme-labeled assay,formation of calcium nodes by Alizarin red staining,and expression ofβ-catenin by Western blotting.Results ADSC-derived exosomes promoted differentiation of ADSCs into osteoblasts by upregulating the expression ofβ-catenin.Addition of Dickkopf-related protein 1(DKK1),an inhibitor of Wnt/β-catenin pathway,inhibited expression ofβ-catenin which in turn resulted in reduced potential of ADSCs for osteogenic differentiation.Conclusion The osteogenic differentiation of ADSCs can be promoted by exosomes derived from ADSCs via the Wnt/β-catenin signaling pathway.

关 键 词：脂肪干细胞 外泌体 WNT/Β-CATENIN信号通路 成骨分化 

分 类 号：R62[医药卫生—整形外科]