基于D-SERS法表征两性霉素B对白色念珠菌抑制作用的研究  

Research on the inhibitory effect of amphotericin B against Candida albicans by D-SERS

作　　者：范加腾 陆峰 许激扬[1] FAN Jiateng;LU Feng;XU Jiyang(College of life science and technology,China Pharmaceutical University,Nanjing 210000,China)

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210000

出　　处：《药学实践杂志》2019年第2期156-161,169,共7页Journal of Pharmaceutical Practice

摘　　要：目的建立动态表面增强拉曼光谱法(D-SERS)快速表征两性霉素B(AMB)对白色念珠菌的抑制作用。方法采用D-SERS法,选用敏感的白色念珠菌分别与不同浓度的AMB相互作用,在不同的作用时间下,通过考察拉曼位移在656 cm^(-1)和729 cm^(-1)处的峰强比和药物浓度之间的关系,分析AMB对白色念珠菌拉曼光谱的影响。结果在一定药物浓度和作用时间下,峰强比与AMB浓度呈负相关,可用于描述AMB对白色念珠菌的抑制作用,且药物浓度低于其最小抑菌浓度。结论该方法简便、快捷,实现了AMB对白色念珠菌的快速表征,同时为表征更多药物-病原体相互作用提供了更简单、快速的检测方法。Objective To develop a rapid method to characterize the inhibitory effect of amphotericin B(AMB)against Candida albicans by dynamic surface enhanced Raman spectroscopy(D-SERS).Methods D-SERS were applied to analyze the effect of AMB on the Raman spectra of C.albicans by examining the relationship between the peak intensity ratio and the concentration of drug at Raman shift at 656 cm-1 and 729 cm-1 under different interaction time by a sensitive C.albicans and different AMB concentrations.Results The peak intensity ratio was negatively correlated with the concentration of AMB,which could be used to describe the inhibitory effect of AMB on C.albicans in a certain concentration and action time.Conclusion This method could characterize the inhibitory effect of AMB against C.albicans rapidly,which provided a simpler and faster detection method for characterizing more drug-pathogen interactions.

关 键 词：白色念珠菌 两性霉素B 动态表面增强拉曼光谱法 

分 类 号：R917[医药卫生—药物分析学]