种子类药材菟丝子提取工艺研究  

Study on extraction process of seed medicinal material Cuscutae Semen

作　　者：夏宇成 张兰 陈宇洁 鞠建明[1] 史艳玲 XIA Yucheng;ZHANG Lan;CHEN Yujie;JU Jianming;SHI Yanling(Department of Pharmaceutical Analysis,Affiliated Hospital of Integrated Traditional Chinese and Western Medicine,Nanjing University of Chinese Medicine,Nanjing,Jiangsu 210028,China;Nanjing No.1 Middle School,Nanjing,Jiangsu 210001,China;Department of Pharmacy, ixing Institute of Dermatology and Venereology,Yixing,Jiangsu 214200,China)

机构地区：[1]南京中医药大学附属中西医结合医院中药分析室,江苏南京210028 [2]南京市第一中学,江苏南京210001 [3]宜兴市皮肤病防治所药剂科,江苏宜兴214200

出　　处：《安徽医药》2019年第4期724-727,共4页Anhui Medical and Pharmaceutical Journal

基　　金：江苏省科技支撑计划--社会发展项目(BE2012776)

摘　　要：目的针对菟丝子种皮致密,提取溶剂不易渗透进入种子内部,有效成分难以提取出来的问题,本实验拟研究该药材的提取方法,优化提取工艺参数。方法以菟丝子中主要活性成分金丝桃苷含量为评价指标,采用单因素平行比较法确定菟丝子提取前处理方法(捣碎与否)、提取方式(包煎与否)和溶剂类型(水或乙醇),采用L_9(3~4)正交试验法进一步优化提取工艺参数。结果包煎不利于有效成分的溶出,种子捣碎有利于有效成分的溶出,菟丝子最适宜的提取工艺为首先将其捣碎破裂开,然后采用不包煎的方式,用10倍量55%乙醇提取2次,每次1 h,验证实验菟丝子中金丝桃苷的提取量为2.04%,RSD(相对标准差)为1.7%。结论优选的菟丝子提取工艺稳定可行,可用于该药材的提取,为同类性质的种子类药材的提取提供参考。Objective To study the extraction method and to optimize the extraction process parameters of Cuscutae Semen according to the problem that the effective ingredients are difficult to extract due to the dense seed coat of Cuscutae Semen and difficult penetration of solvent into the seeds.Methods With contents of hyperoside,one of the main active ingredients of Cuscutae Semen,as index,single factor parallel comparison method was employed to investigate the extraction method including material pretreatment(mashed or not),extraction means(packaged decocting or not)and solvent type(water or ethanol),and L 9(3^4)orthogonal test was employed to optimize the extraction process parameters.Results Packaged decocting extraction method was not conducive to the dissolution of active ingredients;mashi ng the seeds was beneficial to the dissolution of the active ingredients and the optimal extracting process was as follows:mashing the seeds first,decocting twice with 10 times the amount of 55%ethanol for 1 hour each time without package.The average extraction amount of hyperoside in Cuscutae Semen was 2.04%,and RSD was 1.7%in the verification experiment.Conclusion The optimized extraction technology was stable,feasible and suitable for Cuscutae Semen extraction,which provided reference for the extraction of the same kind of seeds.

关 键 词：药用种子植物 菟丝子 金丝桃苷 回收率 煎出率 中药工艺改进 单因素试验 正交试验 

分 类 号：R284.2[医药卫生—中药学]