盐芥的组织培养及抗盐碱性研究  

Study of Tissue Culture and Anti-salinity of Thellungiella salsuginea(Pall.) O.E. Schulz

作　　者：高顺航 张鑫蒙 张洁 杜春雪 王汉海[1] GAO Shun-hang;ZHANG Xin-meng;ZHANG Jie;DU Chun-xue;WANG Han-hai(College of Bio-engineering and Agriculture,Weifang University,Weifang Shandong 261061)

机构地区：[1]潍坊学院生物与农业工程学院,山东潍坊261061

出　　处：《现代农业科技》2019年第5期160-160,165,共2页Modern Agricultural Science and Technology

基　　金：潍坊学院大学生研究训练计划项目(WFUSRTP2017L17)

摘　　要：为研究不同NaCl浓度和pH值对盐芥种子萌发和组培苗生长的影响,探讨其作用机理,本文设置不同NaCl浓度和pH值梯度的MS培养基,测定盐碱胁迫下盐芥种子萌发和组培苗生长的各项指标。结果表明,低盐浓度(50～100 mmol/L)、低pH值(5.0～8.0)下组培苗生长良好,随NaCl浓度升高(>200 mmol/L)和pH值增加,对组培苗生长的抑制作用也逐渐增强。可见,高盐碱可降低盐芥种子萌发率,延缓或抑制盐芥组培苗的生长。In order to study the effects of different NaCl concentrations and pH values on seed germination and tissue culture seedlings growth of The llungie llasals ugine a (Pall.) O.E. Schulz,discuss the mechanism of action,MS medium with different NaCl concentrations and pH gradient was set to determine the various indicators for seed germination and tissue culture seedlings growth of Thellungiella salsuginea (Pall.) O.E. Schulz under saline- alkali stress.The results showed that,the tissue culture seedlings grew well under low salt concentration(50-100 mmol/L)and low pH value(5.0- 8.0).With the increase of NaCl concentration(>200 mmol/L)and pH value,the growth of tissue culture seedlings inhibition also gradually increased. It could be seen that high salinity and alkalinity reduced the germination rate of Thellungiella salsuginea (Pall.) O.E. Schulz seeds and delayed or inhibited the growth of Thellungiella salsuginea (Pall.) O.E. Schulz tissue culture seedlings.

关 键 词：盐芥 组织培养 抗盐碱 继代苗 

分 类 号：Q945[生物学—植物学]