矮壮素对小叶楠抗寒生理的影响  

Effect of Chlormequat on Cold-resistance of Phoebe microphylla

作　　者：费越[1] 白志波 牛学舫 胡蝶[1] 刘刚[1] FEI Yue;BAI Zhi-bo;NIU Xue-fang;HU Die;LIU Gang(Center of Phoebe Germplasm Resources Evaluation and Innovation,Yangtze Uinivesity,Jingzhou,Hubei434025,China;Management Office of Times Square,Langfang,Hebei065000,China)

机构地区：[1]长江大学楠木种质资源评价与创新中心,湖北荆州434025 [2]河北省廊坊市时代广场管理处,河北廊坊065000

出　　处：《福建农业学报》2019年第1期117-123,共7页Fujian Journal of Agricultural Sciences

基　　金：湖北省自然科学基金项目(2017CFB390);湖北省科技支撑计划项目(2013BBB24)

摘　　要：【目的】探讨不同浓度矮壮素(chlormequat chloride,CCC)溶液对小叶楠幼苗叶片组织生理的影响,探究CCC对小叶楠幼苗的抗寒机制,为人工抚育小叶楠提供科学依据。【方法】以小叶楠(Phoebe microphylla)幼苗为材料,采取室内控温系统模拟低温胁迫条件,研究CCC处理对低温胁迫下小叶楠幼苗可溶性蛋白含量(SP)、丙二醛含量(MDA)、相对电导率(REC)、超氧化物歧化酶活性(SOD)、过氧化物酶活性(POD)以及过氧化氢酶活性(CAT)的影响。【结果】低温条件下喷施CCC可使小叶楠幼苗叶片中SP含量增加,喷施50 mg·L^(-1) CCC时,SP含量最高;在0℃和-2.5℃条件下,喷施100 mg·L^(-1) CCC可有效降低MDA含量;喷施50 mg·L^(-1) CCC使小叶楠叶片REC值降低;在-2.5℃时,喷施100 mg·L^(-1) CCC溶液的小叶楠幼苗SOD、POD和CAT活性最强。【结论】通过综合分析各抗寒指标,喷施100 mg·L^(-1) CCC溶液时隶属函数评价最高,说明CCC能有效提高小叶楠的抗寒能力。【Objective】Effect of chlormequat application on low-temperature tolerance of Phoebe microphylla was studied.【Method】Under simulated conditions, seedlings of P. microphylla were subjected to low-temperature stress with treatments of chlormequat chloride (CCC) in different concentrations. The contents of soluble protein (SP) and malondialdehyde (MDA), as well as the relative electrical conductivity (REC) and the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) of the seedlings were determined.【Result】After spraying with CCC solutions, SP in leaves increased and reached a maximum when 50 mg/L of CCC was applied;MDA reduced when 100 mg·L^-1 of CCC was applied at 0℃ or -2.5℃;REC declined at the presence of 50 mg·L^-1 of CCC;and, the activities of SOD, POD and CAT peaked at the spraying of 100 mg·L^-1 of CCC at -2.5℃.【Conclusion】Based on the above mentioned cold resistance indicators, the CCC application on the seedlings at 100 mg·L^-1 scored the highest among all tested treatments on the membership function evaluation. It was considered effective for improving the cold resistance of P. microphylla.

关 键 词：小叶楠 矮壮素 丙二醛 可溶性蛋白 保护酶系统 

分 类 号：S792.24[农业科学—林木遗传育种]