黄芪总皂苷对实验性脑出血大鼠脑水肿与神经功能的影响及作用机制  

E ffects and Mechanism of Total Saponins of Astragalus on Brain Edema and Neurological Function in Experimental Cerebral Hemorrhage Rats

作　　者：郭辉 钟燕 杨贤义 方志成 Guo Hui;Zhong Yan;Yang Xianyi(Taihe Hospital Affiliated to Hubei University of Medicine,Hubei,Shiyan 442000,China)

机构地区：[1]湖北省十堰市太和医院湖北医药学院附属医院,湖北十堰442000

出　　处：《中国中医急症》2019年第3期453-456,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：湖北省十堰市科技局引导性科研项目(2014CFB314)

摘　　要：目的观察黄芪总皂苷对实验性脑出血大鼠脑水肿与神经功能的影响及作用机制。方法将大鼠分为假手术组,模型组和黄芪总皂苷低、中、高剂量组,制备脑出血模型,在造模成功1 d后分别腹腔注射给予50、100、200 mg/kg的黄芪总皂苷溶液,每日1次,持续给予7 d,假手术组和模型组给予等量生理盐水。在实验结束时进行神经功能缺损评分和脑含水量测定,Western blotting法检测脑水肿周围组织中Bax、Bcl-2和Caspase-3蛋白的表达量,TUNEL法检测脑水肿周围组织中细胞凋亡情况。结果给予黄芪总皂苷干预后,与模型组比较,大鼠神经功能评分增高,脑组织含水量降低,差异有统计学意义(P <0.05)。与假手术组比较,模型组大鼠脑水肿周围组织中Bax和Caspase-3蛋白表达水平及细胞凋亡率增加,Bcl-2蛋白表达水平降低,差异有统计学意义(P <0.05);给予黄芪总皂苷干预后,与模型组比较,大鼠脑水肿周围组织中Bax和Caspase-3蛋白表达水平及细胞凋亡率降低,Bcl-2蛋白表达水平增高,且具有一定剂量依赖性,差异有统计学意义(P <0.05)。结论黄芪总皂苷能减轻实验性脑出血大鼠脑水肿和保护神经功能,这种作用可能是通过抑制脑细胞凋亡有关。Objective: To investigate the effect and mechanism of total saponins of astragalus on brain edema and neurological function in experimental cerebral hemorrhage rats. Methods: Rats were divided into sham operation group(normal saline),the model group(normal saline) and total saponins of Astragalus low(50 mg/kg),medium(100 mg/kg) and high dose(200 mg/kg) groups. All the treatment was once a day for 7 days. The neurological defect scores and cerebral tissue moisture content were detected. Moreover,the protein expression levels of Bax,Bcl-2 and Caspase-3 in the surrounding tissues of brain edema were also detected. Thereafter,the cell apoptosis was detected by TUNEL method. Results: Compared with the sham-operated group,the expression of Bax and Caspase-3 protein and apoptotic rate increased,while the expression of Bcl-2 protein decreased in the surrounding tissues of brain edema in the model group;the difference was statistically significant(P < 0.05). After the intervention of astragaloside,compared with the model group,the neurological function score of rats increased and the water content of brain tissue decreased(P < 0.05). The expression level of Bax and Caspase-3 protein and apoptotic rate decreased,while the expression level of Bcl-2 protein increased in rats with brain edema,which was dose-dependent and the difference was statistically significant(P < 0.05). Conclusion: Total saponins of Astragalus membranaceus can alleviate brain edema and protect nerve function in experimental cerebral hemorrhage rats,which may be related to inhibition of apoptosis of brain cells.

关 键 词：黄芪总皂苷 脑水肿 大鼠 神经功能 

分 类 号：R285.5[医药卫生—中药学]