褐黄血蜱卵蛋白质组分析  

Proteomics Analysis of Haemaphysalis flava Eggs

作　　者：徐律 程天印[1,2,3] XU Lü;CHENG Tianyin(College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;Hunan Co-Innovation Center of Animal Production Safety,Changsha 410128,China;Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines,Changsha 410128,China)

机构地区：[1]湖南农业大学动物医学院,长沙410128 [2]湖南畜禽安全生产协同创新中心,长沙410128 [3]兽用蛋白质工程疫苗湖南省重点实验室,长沙410128

出　　处：《畜牧兽医学报》2019年第3期627-636,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：湖南省自然科学基金(2018JJ2167);湖南农业大学"双一流"建设项目(SYL201802016)

摘　　要：本研究旨在探明褐黄血蜱卵内蛋白组分,揭示蜱胚胎发育中的关键营养物质,为筛选可干扰胚胎发育的抗原分子奠定基础。采用液相色谱-串联质谱法对新鲜褐黄血蜱的卵蛋白成分进行分析,基于该蜱的唾液腺转录组翻译文库、中肠转录组翻译文库和Uniprot数据库对各蛋白成分进行鉴定。结果显示:从褐黄血蜱卵黄蛋白提取液中检出特异性肽段221条,由此鉴定蛋白53种,其中高可信蛋白12种,能够确定的功能蛋白有肌动蛋白(actin)和卵黄蛋白原Ⅱ(vitellogenin 2,Vg-2),且Vg-2与卵黄蛋白(vitellins, Vn)一级结构相同。蜱卵原生质所含蛋白较少;Vg-2是蜱卵内存在的唯一一种卵黄蛋白原。The protein components of Haemaphysalis flava eggs were investigated, and the key nutrients were analyzed in the development of embryo, in order to lay a foundation for screening antigen molecules that can interfere with embryonic development. Firstly, the egg protein components of fresh Haemaphysalis flava were analyzed by LC-MS/MS, and based on the salivary gland transcriptome translation library, the midgut transcriptome translation library and the Uniprot database, each protein components were identified. A total of 221 unique peptides were detected from the yolk protein extract of Haemaphysalis flava , and 53 kinds of proteins were identified, among which 12 were highly confident proteins. The functional proteins that can be identified were actin and vitellogenin 2 (Vg-2). Vg-2 is completely same with the primary structure of vitellins (Vn). The protoplasts of the eggs contain less protein, and there is only one type of vitellogenin (Vg-2).

关 键 词：褐黄血蜱 卵 液相色谱-串联质谱 卵黄蛋白原 特异性肽段 

分 类 号：S852.746[农业科学—基础兽医学]