砖红壤微生物总DNA的提取方法比较  

Comparison of Extraction Methods for Total DNA of Microbes from Latosols

作　　者：邓大豪 邓涛 周游[2] 汪军[2] 杨腊英[2] 黄俊生[2] DENG Dahao;DENG tao;ZHOU you;WANG jun;YANG Laying;HUANG Junsheng(Institute of Environment and Plant Protection,CATAS,Haikou,Hainan 571101;Hainan University,Haikou,Hainan 570228)

机构地区：[1]中国热带农业科学院环境与植物保护研究所,海南海口571101 [2]海南大学,海南海口570228

出　　处：《热带农业科学》2019年第1期47-51,共5页Chinese Journal of Tropical Agriculture

基　　金：国家重点研发计划项目(No.2017YFD020060206);海南自然科学基金创新研究团队项目(No.2017CXTD016);中国热带农业科学院基本科研业务费专项资金(No.1630042018013);中国热带农业科学院环境与植物保护研究所自主选题项目(No.hzsjy2017004);广西创新驱动发展专项资金项目(No.桂科AA18118011-2)

摘　　要：针对中国南方砖红土壤的特点,通过比较和优化土壤微生物总DNA的提取方法,获得较高质量的DNA,从而进行土壤细菌16S r DNA基因扩增和真菌I TS r DNA基因扩增。结果表明:方法一提取的土壤微生物DNA受到腐殖质等影响无法进行基因扩增;方法二提取的DNA可以进行土壤微生物基因组16S r DNA基因扩增,提取时间大大减少,但无法进行I TS r DNA基因扩增;方法三提取的DNA质量最好,可以进行细菌16S r DNA和真菌I TS r DNA基因组扩增。According to the c haracteristics of latosols in southern China,extraction methods for soil microbial total DNA were compared and optimized to produce high quality total DNA s from the soil microbes in latosols,and the soil bacteria were identified by using the 16 S r DNA amplification and the soil fungi by using ITS r DNA.The results showed that the soil microbial DNA extracted by the first method could not be amplified by the ITS and 16 s primers because of the existence of humus and others.The DNA extracted by the second method could be used to amplify the 16 S r DNA gene of soil microbial genome with shorter extraction time,but the ITS r DNA gene amplification could not be performed.The extracted DNA by the third method was best in quality and could be used for bacterial and fungal genome amplification.

关 键 词：土壤微生物 DNA提取 16SrDNA ITSrDNA 

分 类 号：Q939.1[生物学—微生物学]