EMS诱变马铃薯抗寒突变体筛选  

Selection of Cold-resistant Variant on Potato by EMS

作　　者：黄萍[1] 李飞[1] 颜谦[1] HUANG Ping;LI Fei;YAN Qian(Potato Research Institute in Guizhou,Guiyang Guizhou 550006,China)

机构地区：[1]贵州省马铃薯研究所,贵州贵阳550006

出　　处：《西南农业学报》2019年第2期241-245,共5页Southwest China Journal of Agricultural Sciences

基　　金：贵州省农业动植物育种专项【黔农育种专字(2014)009】

摘　　要：【目的】定向选择马铃薯抗寒突变体。【方法】以马铃薯品种费乌瑞它为材料,用甲基磺酸乙酯(EMS)诱变处理费乌瑞它愈伤组织,结合添加L-羟脯氨酸(L-Hyp)培养筛选,定向选择抗寒突变体。【结果】诱变费乌瑞它愈伤组织的最佳EMS浓度是0.8%,处理时间4 h;L-Hyp最佳浓度为0.5%。经检测诱变后代再生苗脯氨酸含量明显高出对照,而电解质渗出率均低于对照。【结论】用EMS诱变处理马铃薯愈伤组织可获得抗寒突变体。【Objective】This paper aimed to obtain the cold-resistant variant.【Method】The Favorita potato test-tube plantlet callus could be as materials,then the callus was induced to produce variant by mutagen EMS combination with selection induced callus on L-hydroxyproline(L-Hyp).【Result】The best treatment composition of EMS was 0.8%for 4 hours and the best selecting composition of L-Hyp was 0.5%.Compared to control,the resistant variation and its regenerated plantlet remained characteristic of high proline and low osmotic stress-resisting.【Conclusion】The cold-resistant variant on potato could be obtained by EMS inducing treatment.

关 键 词：EMS L-Hyp 费乌瑞它 愈伤组织 抗寒突变体 

分 类 号：S532[农业科学—作物学]