草莓茎尖脱毒技术研究  

Study on Virus-free Technology of Strawberry Stem Tips

作　　者：聂园军 李瑞珍 张春芬[2] 邓舒[2] 候丽媛 曹秋芬[3] NIE Yuan-jun;LI Rui-zhen;ZHANG Chun-fen;DENG Shu;HOU Li-yuan;CAO Qiu-fen(Institute of Agricultural Resources and Economics,Shanxi Academy of Agricultural Sciences,Taiyuan 030006,China;Fruit Research Institute,Shanxi Academy of Agricultural Sciences,Taigu 030815,China;Biotechnology Research Center,Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China)

机构地区：[1]山西省农业科学院农业资源与经济研究所,山西太原030006 [2]山西省农业科学院果树研究所,山西太谷030815 [3]山西省农业科学院生物技术研究中心,山西太原030031

出　　处：《黑龙江农业科学》2019年第3期29-32,35,共5页Heilongjiang Agricultural Sciences

基　　金：山西省农业科学院特色农业技术攻关项目(YGG17117)

摘　　要：为建立操作简便、脱毒效率高的病毒脱除技术,促进草莓脱毒苗产业化发展,采用感染草莓斑驳病毒的草莓母株为材料,通过对草莓茎尖组织培养法、热培养处理后茎尖组织培养法、组培苗再生匍匐茎茎尖培养法等方式进行脱毒技术研究,比较几种脱毒技术手段的优缺点。结果表明:热处理后,草莓组培苗的脱毒率虽然比直接茎尖培养有所上升,但其成活率却下降。而组培苗再生匍匐茎茎尖培养法与直接茎尖培养法、热培养处理后茎尖培养法相比,在相同茎尖长度时,脱毒率和生存率更高,且茎尖长度为0.8～1.0 mm时,其存活率和成活率达到93.3%和96.4%。因此,组培苗再生匍匐茎茎尖培养法对草莓脱毒是比较有效的方法。In order to establish a simple and efficient virus removal technology and promote the industrialization development of virus-free strawberry seedlings,the virus-free technology of strawberry mother strain infected with strawberry mottle virus was studied by tissue culture of strawberry stem tip,tissue culture of stem tip after heat culture and regeneration of stolon tip of tissue culture seedlings.Advantages and disadvantages were compared.The result showed that the detoxification rate of strawberry tissue culture seedlings increased,but the survival rate decreased after heat treatment.Compared with the direct stem tip culture method and the heat culture method after the stem tip culture,the virus-free rate and survival rate of the stem tip culture method with the same stem tip length were higher,and the survival rate and survival rate reached 93.3%and 96.4%when the stem tip length was 0.8-1.0 mm.Therefore,the regeneration of stolon tip culture with tissue culture was an effective method for strawberry detoxification.

关 键 词：草莓 脱毒技术 匍匐茎再生 

分 类 号：S668.4[农业科学—果树学]