小鼠肝损伤模型研究初探  

Research on Experimental Liver Injury in Mice Model

作　　者：张璐 魏韬[2] 周晶琳[2] 王祥美 白清杰 刘广旭 杨静波 祝慧慧[2] ZHANG Lu;WEI Tao;ZHOU Jinglin;WANG Xiangmei;BAI Qingjie;LIU Guangxu;YANG Jingbo;ZHU Huihui(Clinical Medical College of Mudanjiang Medical University,Mudanjiang 157011,Heilongjiang;Mudanjiang Medical University Basic Medical College,Mudanjiang 157011,Heilongjiang)

机构地区：[1]牡丹江医学院临床医学院,黑龙江牡丹江157011 [2]牡丹江医学院基础医学院,黑龙江牡丹江157011

出　　处：《中国继续医学教育》2019年第8期167-169,共3页China Continuing Medical Education

基　　金：黑龙江省卫生计生委科研课题(2018505);黑龙江省卫生计生委科研课题(2018504);黑龙江省省属高等学校基本科研业务费生物医药类支持计划项目(2018-KYYWFMY-0028);黑龙江省大学生创新创业训练计划项目(201710229041);牡丹江医学院大学生创新创业训练项目(001740);牡丹江医学院教育教学改革项目(MYYB20180010)

摘　　要：目的探讨肝脏部分切除的小鼠模型制备方法,建立简单、安全、有效的肝损伤方法,为筛选抗肝损伤药物提供较为理想的模型。方法明确小鼠肝脏4个解剖功能单位(中叶、左外叶、右叶和尾叶),结扎并切除肝脏左外叶和中叶,建立70%肝切除小鼠模型。结果肝左外叶和中叶约占小鼠肝脏总重量的68%,术后7天小鼠生存状况良好,肝切除小鼠模型成功建立。结论本研究通过建立肝脏部分切除的小鼠模型,探讨部分肝切除小鼠模型方法的安全性和用于后续实验的实用性,以期通过该模型揭示肝脏病变的发生机制,并推动相关研究的发展。Objective Though exploration the method of partial or minor hepatectomy in mice, a simple, safe and effective method for liver injury was established to provide an ideal model for screening anti-liver injury drugs. Methods 4 anatomical functional units(middle, left outer leaves, right and tail leaves) of mice were identified, the left outer leaves and middle leaves of the liver were ligated and removed, and a model of 70 % hepatectomy mice was established. Results The left outer lobe and middle of the liver accounted for 68% of the total liver weight of the mice. The survival condition of the mice was good after 7 days, and the model of hepatectomy mice was established successfully. Conclusion In this study, the safety of partial hepatectomy and the practicability of subsequent experiments were discussed. Moreover, the model will be used to reveal the mechanism of liver injury and promote the development of related studies.

关 键 词：小鼠 肝损伤 肝切除术 动物模型 人类疾病 肝再生 

分 类 号：R3[医药卫生—基础医学]