野菊花提取液中总黄酮含量测定分析  

Determination of total flavonoids of Chrysanthemum indicum extract

作　　者：宋德海[1] 徐倩倩 孙迎东[1] 李保院[1] 郭时金 张金杰[1] SONG Dehai;XU Qianqian;SUN Yingdong;LI Baoyuan;GUO Shijin;ZHANG Jinjie(Department of Pharmacy,Binzhou Medical University Hospital,Binzhou 256603,P.R.China;Binzhou Animal Science and Veterinary Medicine Academy;Lvdu Ante Veterinary Drug Industry)

机构地区：[1]滨州医学院附属医院药学部,滨州256603 [2]滨州市畜牧兽医研究院 [3]绿都安特动物药业有限公司

出　　处：《滨州医学院学报》2019年第1期27-29,共3页Journal of Binzhou Medical University

摘　　要：目的测定野菊花提取液经聚酰胺色谱柱分离后不同洗脱层总黄酮含量。方法采用60%浓度乙醇水加热回流提取野菊花供试品,经聚酰胺色谱柱分离纯化得到不同浓度乙醇洗脱层。采用紫外可见分光光度法对不同洗脱层总黄酮含量进行测定。结果野菊花提取液50%乙醇洗脱液干膏得率为0. 975 3%,干膏得率最高。70%乙醇洗脱层黄酮类化合物含量为46. 51%,含量最高。结论聚酰胺色谱柱分离纯化法准确、灵敏、简便、重复性好,为野菊花黄酮类化合物富集纯化提供了科学基础。ObjectiveTo determine the total flavonoids content of Chrysanthemum indicum extract by polyamide column.MethodsThe sample of Chrysanthemum indicum was heated to reflux by 60%ethanol to get the crude extract.The extract was purified by polyamide chromatography column with different concentrations of ethanol,and the content of the total flavonoids in different eluent layers was determined by UV-Vis spectrophotometry.ResultsThe yield of 50%ethanol eluent in Chrysanthemum indicum extract was 0.9753%and the dry ointment rate was the highest.The content of flavonoids in 70%ethanol eluent was 46.51%and the content was the highest.ConclusionThe method using polyamide chromatography column is accurate,sensitive,simple and reproducible,and it provides a scientific basis for the enrichment and purification of flavonoids of Chrysanthemum indicum.

关 键 词：野菊花 色谱柱 总黄酮 紫外可见分光光度法 

分 类 号：R917[医药卫生—药物分析学]