高效液相色谱法测定林可霉素利多卡因凝胶中盐酸利多卡因含量  

Determination of Lidocaine Hydrochloride in Lincomycin Hydrochloride and Lidocaine Hydrochloride Gel by High Performance Liquid Chromatography

作　　者：欧阳晓玫 牟建平 滕宝霞 贺晓文 OUYANG Xiao-mei;MU Jian-ping;TENG Bao-xia;HE Xiao-wen(Gansu Institute for Drug Control,Lanzhou 730050,China)

机构地区：[1]甘肃省药品检验研究院,甘肃兰州730050

出　　处：《分析测试技术与仪器》2019年第1期39-42,共4页Analysis and Testing Technology and Instruments

摘　　要：建立了高效液相色谱法(HPLC)测定林可霉素利多卡因凝胶中盐酸利多卡因含量的方法.优化后的试验条件如下:色谱柱为Agilent 5 Tc-C18(2)(250 mm×4.6 mm,5μm),流动相为0.05 mol/L硼砂溶液(pH=6.0)-甲醇-乙腈(体积比为68∶19∶13),紫外检测波长为214 nm,流速1.0 mL/min,柱温30℃,进样量20μL.试验结果表明:利多卡因的线性范围为40.1～240.6μg/mL(r=0.999 4),加样回收率为97.6%.建立的方法操作简便,可用于制剂中盐酸利多卡因的质量控制.A method for the determination of lidocaine hydrochloride in lincomycin hydrochloride and lidocaine hydrochloride gel by high performance liquid chromatography(HPLC)is set up.Agilent 5 Tc-C18(2)(250 mm×4.6 mm,5 μm)column was used.The mobile phase consisted of 0.05 mol/L Borax(adjusted to pH 6.0 with phosphoric acid)-methanol-acetonitrile(68∶19∶13).The UV detection wavelength was at 214 nm,with a flow rate of 1 mL/min and the column temperature was 30 ℃.The linearity was good for lidocaine within the range of 40.1~240.6 μg/mL(r =0.999 4),the sample recovery rate was 97.6%.The established method is operative and simple,and can be used for the quality control of lidocaine hydrochloride in preparations.

关 键 词：高效液相色谱法 利多卡因 林可霉素利多卡因凝胶 

分 类 号：O657.72[理学—分析化学]