人腺病毒-B55实时荧光定量PCR扩增方法的建立  

Establishment of fluorescence quantitation PCR to amplify human adenovirus-B55

作　　者：董磊[1] 刘娟[2] 艾现印 马红雨[1] 全首祯[1] 姜涛[4] DONG Lei;LIU Juan;AI Xianyin;MA Hongyu;QUAN Shouzhen;JIANG Tao(Clinical Laboratory Center,the General Hospital of Peoples'Liberation Army Air Force,Beijing 100142,China;Department of Blood Transfusion,the General Hospital of Peoples'Liberation Army Air Force,Beijing 100142,China;Army Health Company 32143 of Chinese People's Liberation Army,Xinyang 464000,Henan,China;Beijing Institute of Microbiology and Epidemiology,State Key Laboratory of Pathogen and Biosecurity,Beijing 100071,China)

机构地区：[1]空军总医院临床检验中心 [2]空军总医院输血科 [3]中国人民解放军32143部队卫生连 [4]军事科学院军事医学研究院微生物流行病研究所

出　　处：《检验医学》2019年第3期259-262,共4页Laboratory Medicine

基　　金：国家科技重大专项(2017ZX10305501);军队后勤科研重大项目(AWS16J020)

摘　　要：目的构建人腺病毒(HAdV)-B55的实时荧光定量聚合酶链反应(PCR)扩增方法,并验证其检测性能。方法采用Beacon Designer 7软件设计针对HAdV-B55 Hexon基因序列的特异性引物,建立HAdV-B55的实时荧光定量PCR扩增方法,并评估其敏感性及特异性。结果建立了检测HAdV-B55的实时荧光定量PCR扩增方法,反应敏感性为0.08 PFU/反应体系,且特异性良好,与相关HAdV B组病毒均未检测到交叉反应。结论成功建立了针对我国HAdV-B55的特异性实时荧光定量PCR检测方法,为HAdV-B55的快速检测及防控提供了有力的支持。Objective To establish real-time fluorescence quantitation polymerase chain reaction(PCR)to amplify human adenovirus(HAdV)-B55,and to verify its performance.Methods The specific primers for the Hexon sequence of HAdV-B55 were designed with Beacon Designer 7 software,and the real-time fluorescence quantitation PCR was established.The sensitivity and specificity were evaluated.Results The specific primers for HAdV-B55 were obtained,and the real-time fluorescence quantitation PCR for HAdV-B55 had been established.The sensitivity was 0.08 PFU/reaction,and the specificity was good.There was no cross reaction with HAdVB Conclusions The real-time fluorescence quantitation PCR to detect HAdV-B55 has been established,which lays the foundation for the rapid detection and prevention of HAdV-B55.

关 键 词：人腺病毒 实时荧光定量聚合酶链反应 快速检测 

分 类 号：R446.1[医药卫生—诊断学]