盐酸小檗碱对2型糖尿病大鼠血管平滑肌细胞增殖、迁移的影响  

Effects of berberine hydrochloride on proliferation and migration of vascular smooth muscle cells in type 2 diabetic rats

作　　者：殷文贤 赵萌 吴知桂 顾立 陈美娟 YIN Wenxian;ZHAO Meng;WU Zhigui;GU Li;CHEN Meijuan(Department of Pharmacy,Affiliated Hospital of Traditional Chinese Medicine,Southwest University,Luzhou,Sichuan 646000,China;Teaching and Researching Section of Pharmacology,Pharmacy College,Southwest Medical University,Luzhou,Sichuan 646000,China)

机构地区：[1]西南医科大学附属中医医院药剂科,四川泸州646000 [2]西南医科大学药理学教研室,四川泸州646000

出　　处：《重庆医学》2019年第5期756-759,764,共5页Chongqing medicine

基　　金：四川省科技厅-泸州市科技局-泸州医学院联合科研项目(14JC0151)

摘　　要：目的观察盐酸小檗碱对2型糖尿病大鼠离体胸主动脉血管平滑肌细胞(VSMLs)增殖、迁移的影响,探究其机制。方法 SD大鼠高脂高糖饮食2个月,于1个月末腹腔注射链脲佐菌素25mg/kg制备2型糖尿病大鼠模型,组织块贴壁法培养胸主动脉VSMLs。CCK-8法观察不同浓度盐酸小檗碱对其增殖的影响、Transwell小室法观察其对迁移的影响、ELISA观察其对转化生长因子β1(TGF-β1)水平的影响。结果 100、50、25、12.5、6.25μg/mL的盐酸小檗碱均可抑制正常和糖尿病大鼠VSMLs的增殖,抑制强度与浓度呈正相关(P<0.01);各浓度盐酸小檗碱均可对正常和糖尿病大鼠VSMLs的迁移产生抑制作用,穿膜细胞数与浓度呈负相关(P<0.01);除6.25μg/mL的盐酸小檗碱外,其余浓度的盐酸小檗碱均可明显降低糖尿病大鼠VSMLs中TGF-β1水平(P<0.05)。结论盐酸小檗碱能抑制2型糖尿病大鼠离体胸主动脉VSMLs增殖、迁移,其机制可能与减少TGF-β1有关。Objective To observe the effects of berberine hydrocloride on the proliferation and migration of vascular smooth muscle cells(VSMLs)of separated thoracic aorta in rats with type 2 diabetes mellitus(T2DM)and to explore its mechanism.Methods The SD rats were fed with 2-month high fat and high sugar diet and conducted intraperitoneal injection of streptozotocin 25 mg/kg at the end of the first month for preparing the T2DM rat model;the tissue block adherence method was used to culture vascular smooth muscle cells of thoracic aorta.The CCK-8 method was used to observe the effect of different concentrations of berberine hydrochloride on proliferation,and the effect of different concentrations of berberine on the migration was observed by Transwell chamber method.The effect of berberine hydrochloride on TGF-β1 level was observed by ELISA.Results 100,50,25,12.5,6.25μg/mL of berberine hydrochloride could inhibit the proliferation of VSMLs in normal and diabetic rats,and the inhibitory intensity was positively correlated with the concentration(P<0.01).Different concentrations of berberine hydrochloride all could produce the inhibiting effect on the migration ofbVSMLs in normal and diabetic rats.The number of transmembrane cells was negatively correlated with the concentration(P<0.01);excepting 6.25μg/mL of berberine hydrochloride,the other concentrations of berberine hydrochloride all could significantly decrease the TGF-β1 level in VMLs of diabetic rats(P<0.05).Conclusion Berberine hydrochloride can inhibit the proliferation and migration of VSMLs of thoracic aorta in T2DM rats,and its mechanism may be related to the decrease of TGF-β1.

关 键 词：盐酸小檗碱 2型糖尿病 大鼠血管平滑肌 增殖 转化生长因子Β1 

分 类 号：R96[医药卫生—药理学]