HPLC法测定植物源杀螨浴剂(外用)中氧化苦参碱的含量  

Determination of Oxymatrine Content in a Botanical Acaricidal Bath Agent for External Use by HPLC

作　　者：刘晓丹 王惠英 李瑞和 常建华 LIU Xiao-dan;WANG Hui-ying;LI Rui-he;CHANG Jian-hua(Inner Mongolia Institute of Veterinary Drug Control,Hohhot 010000,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China)

机构地区：[1]内蒙古自治区兽药监察所,内蒙古呼和浩特010000 [2]内蒙古自治区农牧业科学院,内蒙古呼和浩特010031

出　　处：《畜牧与饲料科学》2019年第3期6-11,共6页Animal Husbandry and Feed Science

基　　金：内蒙古农牧业创新基金项目--植物源动物外用杀虫杀螨剂的研制(2016CXJJM03)

摘　　要：[目的]建立HPLC法测定植物源杀螨浴剂(外用)中的氧化苦参碱含量。[方法]采用Waters XBridge C_(18)(4.6 mm×150 mm,5μm)色谱柱,流动相为甲醇-0.05 mol/L磷酸二氢钠(含0.5%高氯酸钠)(15∶85),流速为1.0 mL/min,检测波长为220 nm,进样量为10μL,柱温为30℃。[结果]氧化苦参碱质量浓度在0.020 592～0.457 47 mg/mL(Y=5.72e+006X+1.13e+004,R2=0.9998)与峰面积积分值呈良好的线性关系;精密度试验RSD为0.3%(n=6);重复性试验RSD为1.0%(n=6);回收率试验RSD为1.6%。[结论]该法测定氧化苦参碱含量简便、快捷,稳定性好,重复性高,可用于控制植物源杀螨浴剂(外用)的质量。[Objective] To establish a HPLC method for determination of oxymatrine content in a botanical acaricidal bath agent for external use.[Method] The separation was performed with the commercially available chromatographic column of Waters XBridge C18 (4.6 mm×150 mm,5 μm).Methanol-0.05 mol/L sodium dihydrogen phosphate (containing 0.5% sodium perchlorate)(15:85) was used as mobile phase,the flow rate was 1.0 mL/min,the detection wavelength was 220 nm,the sample volume was 10 μL,and the column temperature was 30 ℃.[Result]There was a good linearity between the peak areas and the mass concentrations of oxymatrine in the range of 0.020 592 - 0.457 47 mg/mL (Y=5.72e+006X+1.13e+004,R^2=0.9998).The RSD of the precision test (n=6),repeatability test (n=6) and recovery test was 0.3%,1.0% and 1.6%,respectively.[Conclusion] The established HPLC method has the advantages of simplicity,speed,good stability and high repeatability,and can be used in quality control of the botanical acaricidal bath agent for external use.

关 键 词：HPLC 植物源杀螨浴剂(外用) 氧化苦参碱 含量测定 

分 类 号：S859.795[农业科学—临床兽医学]