不同品种桑树根际土壤细菌多样性的高通量测序分析  

High throughput sequencing analysis of rhizosphere soil bacteria diversity in different mulberry varieties

作　　者：邱洁 侯怡铃[1] 徐丽丽 钱叶[1] 丁祥 QIU Jie;HOU Yi-ling;XU Li-li;QIAN Ye;DING Xiang(College of Life Sciences,ChinaWest Normal University/Key Laboratory of Southwest ChinaWildlife Resources Conservation,Ministry of Education,Nanchong,Sichuan 637009,China;College of Environmental Science and Engineering,ChinaWest Normal University,Nanchong,Sichuan 637009,China)

机构地区：[1]西华师范大学生命科学学院/西南野生动植物资源保护省部共建教育部重点实验室,四川南充637009 [2]西华师范大学环境科学与工程学院,四川南充637009

出　　处：《南方农业学报》2019年第3期585-592,共8页Journal of Southern Agriculture

基　　金：四川省财政创新能力提升专项项目(2016ZYPZ-030);"十三五"四川省农作物育种项目(2016NYZ0041-01);四川省农业科技成果转化项目(2016NZ0074);国家级大学生创新训练项目(201610638045)

摘　　要：【目的】探究不同品种桑树根际土壤细菌群落结构及其分布规律,为促进桑树的生长发育、提高其经济利用价值和培育更高品质的桑树品种提供参考。【方法】对3个品种桑树(粤椹大10、嘉陵30号和红果1号)根际土壤细菌的16S rDNA序列V3～V5高变区进行PCR扩增,并对PCR扩增产物进行高通量测序,分析土壤中细菌群落多样性及分布规律。【结果】3个土壤样品中共检测出细菌26门76纲88目149科370属。不同品种桑树根际土壤样品中的细菌群落组成和结构存在一定差异,丰富度方面表现为嘉陵30号>粤椹大10>红果1号;多样性方面表现为红果1号>嘉陵30号>粤椹大10。3个品种桑树的根际土壤细菌在门纲目科属水平上的优势菌群及所占比例分别为变形菌门(Proteobacteria,37.2%)、α-变形菌纲(Alphaproteobacteria,24.3%)、拟杆菌目(Bacteroidales,24.3%)、拟杆菌科(Bacteroidaceae,14.2%)、拟杆菌属(Bacteroides,14.2%);随着分类的细化,不同桑树品种对土壤细菌群落组成和分布的影响越大。【结论】变形菌门在3种桑树根际土壤中均是最优势细菌类群,嘉陵30号桑树品种根际土壤细菌种类最多,红果1号桑树品种土壤细菌分布最均匀。根际土壤细菌群落种类和均匀度可作为桑树差异规模化种植的指标。【Objective】The purpose of the study was to explore the structure and distribution of rhizosphere soil bacterial community of different mulberry varieties,provide scientific basis for promoting the growth and development of mulberry,improving the economic value of mulberry and cultivating mulberry varieties with high quality.【Method】The 16S rDNA sequence V3-V5 high-variable region of rhizosphere soil bacteria in three mulberry varieties(Yueshenda 10,Jialing 30 and Hongguo 1)was amplified by PCR,and then the products of PCR amplification were sequenced with high throughput to analyze the diversity and distribution of bacterial community in soil.【Result】A total of 370 genera,149 families,88 orders,76 classes and 26 phyla of bacteria were detected in the three soil samples.There were some differences in the structure and composition of bacterial community in rhizosphere soil samples of different mulberry varieties.In terms of richness,Jialing 30>Yueshenda 10>Hongguo 1,while in terms of diversity,Hongguo 1>Jialing 30>Yueshenda 10.The dominant bacterial community of rhizosphere soil bacteria of three mulberry varieties and their proportions were Proteobacteria(37.2%),Alphaproteobacteria(24.3%),Bacteroidales(24.3%)and Bacteroidaceae(14.2%),Bacteroides(14.2%).However,with the refinement of classification,the influence of mulberry varieties on the composition and distribution of soil bacterial community became great.【Conclusion】Proteobacteria is the most abundant bacterial group in three mulberry rhizosphere soils.The rhizosphere soil bacteria of Jialing 30 are the most abundant,and Hongguo 1 has the most uniform distribution of soil bacteria.The species and evenness of bacterial community in the rhizosphere soil can be used as indicators for mulberry differential large-scale planting.

关 键 词：桑树 根际土壤 细菌 多样性 高通量测序 

分 类 号：S888.5[农业科学—特种经济动物饲养]