硫杂杯[4]芳烃基胶束自组装荧光探针的结构性质及检测性能的研究  

Study on the Structural Properties and the Detection Performances of Thiacalix[4]arene-Based Micellar Self-Assembled Fluorescent Probe

作　　者：李园义 汪波 张滢 胡晓钧 张智 胡新妍 LI Yuan-yi;WANG Bo;ZHANG Ying;HU Xiao-jun;ZHANG Zhi;HU Xin-yan(School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China)

机构地区：[1]上海应用技术大学化学与环境工程学院,上海201418

出　　处：《光谱学与光谱分析》2019年第4期1092-1096,共5页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金面上项目(21277093);上海市曙光计划(17SG52);上海市科技计划(16090503500);上海应用技术大学科研启动项目(YJ2016-36)资助

摘　　要：水体中重金属污染因威胁生态环境和人类健康而被受广泛关注。荧光探针由于具有快速高效检测重金属的特性,一直是该领域的研究热点。通常,荧光探针在结构上包括对待测物质起识别作用的受体和能产生信号响应的荧光体,并逐步形成了内在型、共轭型、系综型和模板辅助自组装型等四种结构类型。近年来,基于受体和荧光体在表面活性剂胶束内自组装而形成的胶束自组装型荧光探针因结构简单、易于制备、能直接应用于水环境等特点逐渐受到重视。以对铜离子具有优异结合性能的对叔丁基硫杂杯[4]芳烃(TCA)为受体,以芘、荧蒽、蒽、菲、苝等分子为荧光体,通过表面活性剂胶束自组装制备针对Cu^(2+)检测的胶束自组装型荧光探针,采用参比法测定了胶束自组装荧光探针的荧光量子产率,采用稳态荧光法测定了胶束聚集数,同时通过计算荧光猝灭率分别考察了荧光体种类、复配表面活性剂等因素对该探针的Cu^(2+)检测性能的影响情况。实验结果显示,采用十二烷基硫酸钠(SDS)、曲拉通100(TX-100)、聚氧乙烯月桂醚(Brij35)等三种不同的表面活性剂对探针荧光体的荧光量子产率产生了明显影响,测得的荧光探针荧光量子产率介于0.25～0.47,且三者逐渐增大,说明表面活性剂改变了胶束内荧光分子芘所处微环境的极性,且不同类型表面活性剂对微环境极性的影响程度有所差异,微环境极性的增强对极性更大的激发态芘具有更强的稳定作用。而受体TCA的加入对荧光体所处微环境极性影响较小,未对荧光量子产率产生较大影响。但TCA的加入使探针的胶束聚集数明显减少,这归因于具有两亲性的受体TCA分子通过胶束自组装进入并分散在表面活性剂分子层中,形成共胶束结构,从而改变了表面活性剂分子的聚集状态。荧光体变更对荧光探针的Cu^(2+)检测性能有显著影响,在�Heavy metal pollution in water is widely concerned because it threatens the ecological environment and human health. The fluorescent probe has been a research focus in this field due to the rapid and efficient detection for heavy metals. Generally, the fluorescent probe structurally includes a receptor recognizing a desired analyte and a fluorophore generating a signal response. It gradually has formed four kinds of structures, which are intrinsic, conjugate, ensembling and template-assisted self-assembled types. In recent years, micellar self-assembled fluorescent probes based on the self-assembly of acceptor and fluorophore in surfactant micelles have attracted attentions. This is due to their simple structure, easy preparation and direct application to water environment. In this paper, the micellar self-assembled fluorescent probes for the detection of Cu^2+ ions were prepared through self-assembly of surfactant micelles. The p-tert-butylthiacalix[4]arene(TCA) was used as acceptor with excellent bonding property to copper ions. And pyrene, fluoranthene, anthracene, phenanthrene, perylene were used as fluorophore. The fluorescence quantum yields of the micellar self-assembled fluorescent probes were measured by the reference method. The micelle aggregation numbers were determined by the steady-state fluorescence method. At the same time, the influences of fluorophore species and compound surfactants were investigated on detection performances of the probes for Cu^2+ ions by calculating the fluorescence quenching rate. The experimental results showed that the three surfactants, which are sodium dodecyl sulfate(SDS), Triton X-100(TX-100) and polyoxyethylene lauryl ether(Brij35), had significant effects on fluorescence quantum yields of the probes. Their fluorescence quantum yields were in the range of 0.25~0.47. And they gradually increased. These indicated that the polarities of the microenvironment inside the micelles were changed by surfactant micelles. And the influences of different types of surfactants on t

关 键 词：硫杂杯[4]芳烃 胶束自组装 荧光探针 结构性质 检测性能 

分 类 号：O657.3[理学—分析化学]