丹参川芎嗪对离体大鼠心肌缺血/再灌注损伤的保护作用  

Protective effects of ligustrazine of salvia miltiorrhiza on myocardial ischemia/reperfusion injury in isolated rat

作　　者：黄文东 杨艳芳 高琦 李启森 朱邦豪 HUANG Wendong;YANG Yanfang;GAO Qi;LI Qisen;ZHU Banghao(Pharmacy Department,Maoming people’s hospital,Maoming 525000,China;Central laboratory,Maoming people’s hospital,Maoming 525000,China;Cardiovascular and cerebrovascular research center,Sun Yat-sen Medical College,Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]广东省茂名市人民医院药剂科,广东茂名525000 [2]中山大学中山医学院心脑血管研究中心,广东广州510080 [3]广东省茂名市人民医院中心实验室,广东茂名525000

出　　处：《中国医药科学》2019年第5期9-13,共5页China Medicine And Pharmacy

基　　金：广东省医学科学技术研究基金项目(A2017490)

摘　　要：目的通过对大鼠离体心脏灌流不同剂量受试药丹参川芎嗪(SLI),探讨受试药对离体心肌缺血/再灌注(I/R)损伤的保护作用。方法采用Langendorff离体心脏恒压灌流系统建立心肌缺血/再灌注模型。SD大鼠随机分为五组(n=11),包括空白对照组(Control)、模型组(Model)、SLI高剂量组(High)、中剂量组(Middle)、低剂量组(Low)。将分离后的大鼠心脏连接在Langendorff离体灌流仪器上,先平衡灌注20min,再全心停灌20min,然后复灌60min;空白对照组连续灌流100min。采用labchart生物信号采集仪器记录观察各受试药物组对心功能的影响。结果与模型组相比,SLI各剂量组I/R的相关指标LVDP、+dp/dtmax明显升高(P <0.05),冠脉流出液中LDH、CK、MDA含量显著降低(P <0.05),而SOD水平则明显升高。HE染色结果显示,模型组心肌细胞着色不均、纤维排列紊乱、心肌形态不完整,心肌纤维间可见中性粒细胞浸润,部分心肌细胞坏死,而SLI各剂量组均不同程度地改善了这一改变。结论 SLI能减轻离体心肌缺血/再灌注诱导的冠脉损伤,对心脏功能恢复具有促进作用,其机制可能与改善心肌舒缩功能,清除氧自由基有关。Objective To investigate the protective effect of the drug on isolated cardiac ischemia/reperfusion(I/R)injury by salvia miltiorrhiza and ligustrazine injection(SLI) on isolated myocardial ischemia/reperfusion injury in rats. Methods Langendorff isolated cardiac constant pressure perfusion system was used to establish the myocardial ischemia/reperfusion model.SD rats were randomly divided into 5 groups(n=11),including control group,model group,SLI group,middle group and low group.The separated rat hearts were connected to the Langendorff isolated perfusion apparatus.The rat hearts were placed in equilibrium perfusion for 20min,then completely stopped perfusion for 20min,and then re-perfusion for 60min. The blank control group was given continuous perfusion for 100min.Labchart biological signal acquisition instrument was used to record and observe the effect of each drug group on cardiac function. Results Compared with the model group,the SLI dose groups had significantly higher I/R related indicators of LVDP and +dp/dtmax(P<0.05).The contents of LDH,CK and MDA in coronary effluent were significantly decreased(P<0.05),while the levels of SOD were significantly increased. The results of HE staining showed that the model group had uneven staining of myocardial cells,disorganized arrangement of fibers,and incomplete myocardial morphology,with neutrophil infiltration among myocardial fibers and necrosis of some myocardial cells,and the SLI dose groups improved this change to varying degrees. Conclusion SLI can alleviate isolated ischemia/reperfusion induced coronary artery injury and promote cardiac function recovery,and its mechanism may be related to improving myocardial systolic function and scavenging oxygen free radicals.

关 键 词：丹参川芎嗪 LANGENDORFF 心脏血流动力学 心肌缺血/再灌注 

分 类 号：R542.2[医药卫生—心血管疾病]