基于PARMS技术的水稻粒形基因GW8分子标记的开发  

Developing Molecular Marker of Rice Grain Shape Gene GW8 Based on PARMS Technology

作　　者：卿冬进 刘开强[2] 邓国富[2] 高利军[1] 黄娟 高菊 伍豪 戴高兴[3] 梁海福[3] 周维永[3] QING Dong-jin;LIU Kai-qiang;DENG Guo-fu;GAO Li-jun;HUANG Juan;GAO Ju;WU Hao;DAI Gao-xing;LliVNG Hai-fu;ZHOU Wei-yong(Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Nanning 530007 , China;Guangxi Academy of A gricultural Sciences, Guangxi Nanning 530007 , China;Rice Research Institute, Guangxi Academy of Agricultural Sciences, Guangxi Nanning 530007 , China)

机构地区：[1]广西作物遗传改良生物技术重点开放实验室,广西南宁530007 [2]广西农业科学院,广西南宁530007 [3]广西农业科学院水稻研究所,广西南宁530007

出　　处：《西南农业学报》2019年第3期463-469,共7页Southwest China Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(31460344);广西农业科学院基本科研业务专项项目(桂农科2017JZ40);广西农业科学院科技发展基金项目(桂农科2015JZ18;桂农科2018JZ34);广西自然科学基金项目(2017GXNSFAA198266)

摘　　要：【目的】开发水稻粒宽调控基因GW8的荧光分子标记,为快速、准确鉴定其基因型提供技术支持。【方法】通过与宽粒亲本GW8基因序列进行比对可知,窄粒杂交稻亲本美B的GW8基因序列在第二内含子有2个碱基缺失差异的特性之一,据此结合五引物扩增受阻突变体系技术,建立GW8基因荧光分子标记PM-GW8。【结果】利用荧光分子标记PM-GW8对42份籼稻亲本材料进行鉴定,仅在美B中检测到有2个碱基缺失的荧光信号。对亲本材料的谷粒宽度进行测量,其中美B的粒宽相对其他品种较窄,验证了该分子标记检测GW8的基因型与粒宽表型相符合。【结论】GW8荧光分子标记PM-GW8能快速检测水稻是否存在2个碱基缺失的GW8基因,为利用分子标记辅助选择改良水稻粒宽提供高效、可靠的基因分型技术。【Objective】In order to provide technical support for efficient and accurate genotyping, the fluorescence molecular markers for rice grain width regulation gene GW8 was developed.【Method】Based on one of the characters of two bases deletion on the second intron of GW8 gene in narrow grain hybrid rice parent MeiB by comparison with wide gain varieties, and combined with penta-primer amplification refractory mutation system, the fluorescence molecular marker PM-GW8 of GW8 gene was developed.【Result】42 indica rice varieties were tested by the PM-GW8 marker, data showed that fluorescence signal of 2 bases deletion of GW8 was only detected in MeiB. These varieties were also analysis with grain width measurement, grain of MeiB was more narrow than grain of other varieties, this result indicated that genotype of GW8 detected by the marker was corresponding to phenotype of grain width.【Conclusion】The fluorescence molecular marker PM-GW8 can be used to detect whether two bases deletion in GW8 gene exist in rice rapidly, providing an efficient and reliable gene screening technic for the use of marker-assisted approach to improve rice gain width.

关 键 词：水稻 粒形 基因 分子标记 

分 类 号：S511[农业科学—作物学]