禽巴氏杆菌四环素耐药株转录组测序与分析  

TRANSCRIPTOME SEQUENCING ANALYSIS OF TETRACYCLINERESISTANT STRAINS OF AVIAN PASTEURELLA MULTOCIDA

作　　者：孔令严[1,2] 汪最 邵华斌 张腾飞[1,2] 卢琴 罗青平[1,2] KONG Ling-yan;WANG Zui;SHAO Hua-bin;ZHANG Teng-fei;LU Qin;LUO Qing-ping(Institute of Animal Husbandry and Veterinary,Hubei Academy of Agricultural Sciences,Wuhan 430064,China;Key Laboratory ofAnimal Disease Control and Prevention Formulation of Ministry of Agriculture,Wuhan 430064,China)

机构地区：[1]湖北省农业科学院畜牧兽医研究所,武汉430064 [2]农业部畜禽细菌病防治制剂创制重点实验室,武汉430064

出　　处：《中国动物传染病学报》2019年第1期30-36,共7页Chinese Journal of Animal Infectious Diseases

基　　金：公益性农业行业科研专项;家禽主要细菌病防控技术研究与示范(201303040);现代农业产业技术体系建设专项资金资助(CARS-42-G11);规模化养鸡场主要疫病防控关键技术研究(2015ABA039)

摘　　要：为鉴定禽源多杀性巴氏杆菌(avian Pasteurella multocida,Pm)四环素(Tetracycline,Tet)敏感株与耐药株(MIC=32μg/mL)在转录组水平上的差异,本研究采用RNA-seq技术对Tet敏感株与耐药株进行双向测序,通过GO、KEGG和ARDB数据库对表达差异基因进行注释和富集性分析。结果显示,共筛选出442个差异表达基因,其中包括103个上调基因和339个下调基因。通过GO功能富集分析显示差异表达基因多数富集于核糖体结构成分、rRNA结合、跨膜运输和离子转运等生物过程;对差异表达基因进行信号通路富集性分析,大多数差异表达基因分布在ABC转运蛋白与核糖体代谢途径中;对差异表达基因进行耐药基因注释(ARDB),发现其中的耐药基因主要为核糖体保护蛋白和外排系统两类。本研究表明禽源Pm对四环素耐药的耐药机制可能与细胞膜上ABC转运蛋白的过量表达以及核糖体保护蛋白有关。To identify the differentially expressed genes of Tetracycline sensitive strains and resistant strains (MIC=32μg/mL) of avian P. multocida, the transcriptomes were detected by high-throughput RNA sequencing. All of differentially expressed genes were enriched and annotated by Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and Antibiotic Resistance Genes Database (ARBD), respectively. The results showed that 442 differentially expressed genes were identified, including 103 up-regulated genes and 339 down-regulated genes. The GO enrichment analysis showed that the majority of differentially expressed genes play a role in “stmctoal constituent of ribosome”,“rRNA binding”,“transmembrane transport”iod “ioo transport”.M ost of the differentially expressed genes were distributed in the “ABC transporters” and “Ribosome” by KEGG analysis. The differentially expressed resistance genes were mainly divided into ribosomal protection protein and efflux pump system by ARDB analysis. In summary, ABC transporters and aminoglycoside transferase overexpression may play important roles in the mechanisms of Tet-resistance in avian multocida.

关 键 词：禽源多杀性巴氏杆菌 转录组测序 GO功能分析 KEGG功能分析 ARDB注释 

分 类 号：S852.612[农业科学—基础兽医学]