华东地区沙门菌流行病学及耐药性分析  

EPIDEMIOLOGY AND ANTIBIOTIC RESISTANCE OF SALMONELLA SPECIES IN EASTERN CHINA

作　　者：吴晓君 王少辉[2] 杨登辉[2] 王栋[2] 田明星[2] 丁铲 高崧[1] 于圣青[2] WU Xiao-jun;WANG Shao-hui;YANG Deng-hui;WANG Dong;TIAN Ming-xing;DING Chan;GAO Song;YU Sheng-qing(College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China;Shanghai Veterinary Research Institute, CAAS,Shanghai 200241, China)

机构地区：[1]扬州大学兽医学院,扬州225009 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2019年第1期49-54,共6页Chinese Journal of Animal Infectious Diseases

基　　金：公益性农业(科研)专项项目(201403054);国家重点研发计划项目(2016YFD0500800);国家自然科学基金项目(31572523);上海市科技兴农重点攻关项目(沪农科攻字(2015)第1-9号)

摘　　要：沙门菌病是一种严重危害人类健康和养殖业发展的人畜共患病,为了解华东地区沙门菌的分子流行情况,本研究采用选择性培养基和PCR分离鉴定沙门菌,然后利用PCR检测沙门菌临床分离株的血清型及毒力因子,并用药敏纸片法检测其耐药性。结果显示,本研究分离鉴定出48株沙门菌,分离率为16.84%。血清学结果显示所检测的48株沙门菌中鼠伤寒沙门菌占64.58%,肠炎沙门菌占6.25%,其他血清型占29.17%。毒力基因检测发现除sopE基因分布率为25%外,其他15个毒力基因分布率可达97%以上。药敏试验结果显示沙门菌分离株对常见抗生素具有不同的抗性和敏感性,并且耐药性十分严重,对红霉素、阿奇霉素、克林霉素、新霉素、大观霉素、四环素、利福平、复方新诺明等抗生素的耐药率均超过50%以上。检测结果说明,华东地区沙门菌主要血清型为鼠伤寒沙门菌,且毒力基因分布广泛,多重耐药性菌株十分流行。Salmonella is an important human and animal pathogen that seriously affects human health and animal industry. Moreover, it is a leading etiological agent associated with food-bome illness outbreaks worldwide, resulting in heavy economic losses globally. To investigate the prevalence of Salmonella species in the Eastern China, bacteria were isolated and identified by PCR and their serotypes and virulence genes were also tested by PCR. The antibiotics susceptibility of the Salmonella isolates were performed in accordance to the routine methods. The results showed that 48 isolates were identified as Salmonella from 285 samples, which accounted for 16.84%. Serological testing revealed that 64.58% isolates belonged to 5. typhimurium and 6.26% isolates to S. enteritidis. The results also showed that the virulence genes were prevalent in over 97% isolates while the sopE gene was present in 25% isolates. These Salmonella isolates were multiple resistances to most antibiotics. More than 50% Salmonella isolates were resistant to erythromycin, azithromycin,clindamycin, neomycin, spectinomycin, tetracyclines, rifampin and compound sulfamethoxazole. This study revealed that S. typhimurimn was the predominant serotype present on farms and these isolates possessed many virulence genes and showed resistance to a number of drugs. Thus, these results suggested the necessity to inspect and survey the Salmonella contamination on farms and in abattoirs to reduce the incidence of foodbome infections in humans.

关 键 词：沙门菌 血清型 毒力基因 耐药性 

分 类 号：S852.612[农业科学—基础兽医学]