镰形扇头蜱卵巢中丝氨酸蛋白酶抑制分子群的克隆和生物信息学分析  

CLONING AND BIOINFORMATICS ANALYSIS OF SERINE PROTEASE INHIBITORS FROM OVARY OF RHIPICEPHALUS HAEMAPHYSALOIDES

作　　者：许正茂 周勇志[2] 曹杰[2] 张厚双[2] 龚海燕[2] 巴音查汗[1] 周金林[2] XU Zheng-mao;ZHOU Yong-zhi;CAO Jie;ZHANG Hou-shuang;GONG Hai-yan;BAYINCHAHAN;ZHOU Jin-lin(College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China;Shanghai Veterinary Research Institute,CAAS, Shanghai 200241, China)

机构地区：[1]新疆农业大学动物医学学院,乌鲁木齐830052 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2019年第1期55-62,共8页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金资助(31572512;31660711)

摘　　要：丝氨酸蛋白酶抑制分子(serine protease inhibitor,Serpin)参与多种生物学过程,对蜱的吸血发育、生殖产卵和先天免疫系统反应等具有重要作用,在蜱体内以多分子形式存在。为了研究蜱生殖相关的Serpin分子群,本研究通过构建镰形扇头蜱(Rhipicephalus haemaphysaloides)卵巢转录组测序数据库,发现并克隆了10条Serpin家族基因(RhS),其中3条为分泌型Serpin,6条为细胞内Serpin和1条亲缘关系较远的Serpin。本研究通过生物信息学方法分析其理化性质、遗传进化关系,预测其结构和功能,为镰形扇头蜱Serpin分子群的功能解析和应用研究提供了重要基础。Serine protease inhibitor (Serpin) participates in a variety of biological functions and plays an important role in blood meal and development, reproductive spawning and innate immune system responses of ticks, and exists in multi-molecule form in ticks. In order to study the population of Serpin related to tick reproduction, 10 Serpin family genes (RhS) were found and cloned by ovarian transcriptome sequencing database of Rhipicephalas haemaphysaloides, of which 3 were secreted Serpin, 6 were intracellular Serpin and a Serpin with a distant relationship. Bioinformatics analysis of its physical and chemical properties, genetic evolution relationship, predict its structure and function, provide important basis for functional analysis and application of Serpin molecular cluster of Rhipicephalas haemaphysaloides.

关 键 词：丝氨酸蛋白酶抑制分子 镰形扇头蜱 克隆 生物信息学 

分 类 号：S852.746[农业科学—基础兽医学]