抗球虫新药沙咪珠利与鸡血浆蛋白结合率的测定  

BINDING RATE OF CHICKEN PLASMA PROTEIN WITH ETHANAMIZURIL

作　　者：程晓蕾 陈桂平 王霄旸[1] 王春梅[1] 刘迎春[1] 薛飞群[1] 王米[1] 张可煜[1] CHENG Xiao-lei;CHEN Gui-ping;WANG Xiao-yang;WANG Chun-mei;LIU Ying-chun;XUE Fei-qun;WANG Mi;ZHANG Ke-yu(Key Laboratory of Veterinary Chemical Drugs and Pharmaceutics,Ministry of Agriculture and Rural Affairs,Shanghai VeterinaryResearch Institute,CAAS,Shanghai 200241,China;Institute of Apicultural Research of Jiangxi Province,Nanchang330052,China)

机构地区：[1]中国农业科学院上海兽医研究所农业农村部兽用化学药物及制剂学重点实验室,上海200241 [2]江西省养蜂研究所,南昌330052

出　　处：《中国动物传染病学报》2019年第1期78-84,共7页Chinese Journal of Animal Infectious Diseases

基　　金：国家科技支撑计划项目(2015BAD11B00);公益性行业(农业)科研专项(201303038);上海自然科学基金项目(14ZR1449000)

摘　　要：为研究抗球虫新药沙咪珠利与鸡血浆中蛋白结合情况,采用平衡透析法模拟沙咪珠利与鸡血浆蛋白结合过程,高效液相色谱法测定透析内液及外液中药物浓度,计算沙咪珠利血浆蛋白结合率。结果表明,鸡血浆中其他成分对测定无干扰,各待测物在选择浓度范围内线性关系均良好,透析外液线性范围为0.1～10μg/mL,透析内液的线性范围为0.5～100μg/mL,提取回收率在94.35%～111.8%之间,日内、日间变异均小于10%,精密度及稳定性结果均符合方法学要求。在设定的3个药物浓度下,沙咪珠利在鸡血浆中的蛋白结合率为92.17%～97.72%,属高血浆蛋白结合率药物,且具有一定的浓度依赖性。In the present study, the equilibrium dialysis was used to imitate the binding process between ethanamizuril and chicken plasma protein. The HPLC was used to determine ethanamizuril in plasma. The results showed that there was no interference from other ingredients for the determination of ethanamizuril. The calibration curve was in good linearity in certain ranges of contents. The precision and stability met the requirements for methodology. The calibration curves were linear in the range of 0.1-10 (μ/mL for the buffer solution and 0.5-100 μ/mL for the plasma. In addition, the extract recovery was 94.35%-111.8% and RSDs of infra and inter - day precisions were all less than 10%. The HPLC method established in this paper was simple, sensitive and stable. In the low, medium and high concentrations, the in vitro binding rates of plasma protein with ethanamizuril were 92.17%-97.72%. In conclusion, ethanamizuril had a high protein binding rate with chicken plasma and this action was concentration dependent.

关 键 词：沙咪珠利 血浆蛋白结合率 高效液相色谱法 鸡 

分 类 号：S859.795[农业科学—临床兽医学]