水稻根际固氮菌分离及最适培养条件研究  

Isolation and Studies of Optimum Growth Condition of Nitrogen Fixation Bacteria in Rice

作　　者：葛江丽[1] 施汉钰[1] 刘桂棋 刘芳[1] 曹昊[1] GE Jiangli;SHI Hanyu;LIU Guiqi;LIU Fang;CAO Hao(Mudanjiang Institute of Forestry Science of Heilongjiang Province,Mudanjiang 157009,China)

机构地区：[1]黑龙江省牡丹江林业科学研究所,黑龙江牡丹江157009

出　　处：《东北农业科学》2018年第4期53-56,共4页Journal of Northeast Agricultural Sciences

基　　金：黑龙江省森工总局应用研究项目(sgzjY2015004)

摘　　要：本研究从水稻根际土壤中筛选高效固氮菌,通过乙炔还原法选出一株固氮能力较强的菌株G3,其固氮酶活性为1.875μmol·ml-1·h-1。通过革兰氏染色和形态观察发现,G3为革兰氏阴性菌。对G3的最适培养条件进行研究,结果表明:G3生长的最适温度是35℃;最适生长pH是7;1%的NaCl为G3的最佳生长浓度;当铵离子浓度达到1 mmol/L时,能够很好地生长。High efficiency nitrogen fixation bacteria were isolated from rice soil.The most effective strain G3 was selected(1.875 μmol·ml^-1·h^-1)by acetylene reduction method.Based on the morphological characters,G3 strain wasgram negative bacteria.The optimal growth of G3 was studied.The experimental results showed that the optimal temperature and pH were 35℃ and 7,respectively.The optimal concentration of NaCl was 1%,and optimal NH4^+was 1 mmol/L.

关 键 词：水稻 固氮菌 分离 最适培养条件 

分 类 号：S511[农业科学—作物学]