苍术白虎汤对高尿酸血症性肾病小鼠作用机制的研究  

Study on the Mechanism of Cangzhu Baihu Decoction for Hyperuricemic Nephropathy Mice

作　　者：李宇轩[1] 宋林 于学成 王相云 杨帆[1] 韩晓春[1,2] Li Yuxuan;SONG Lin;YU Xuecheng(College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine Jinan Shandong 250355,China;College of Pharmacy,Shandong University of Traditional Chinese Medicine Jinan Shangdong 250355,China)

机构地区：[1]山东中医药大学 [2]山东省中医经典名方协同创新中心,山东济南250355

出　　处：《四川中医》2019年第2期44-47,共4页Journal of Sichuan of Traditional Chinese Medicine

基　　金：山东省重点产业关键技术项目(编号:2016CYJS08A01-4;2016CYJS08A01-3);山东省重点研发计划(编号:2016ZDJS07A12);山东中医药大学大学生研究训练计划(SRT)国家级项目(编号:201610441025)

摘　　要：目的:观察苍术白虎汤对高尿酸血症性肾病模型小鼠的影响并探索其机制。方法:采用腺嘌呤(100mg·kg^(-1))联合乙胺丁醇(250mg·kg^(-1))法制备小鼠高尿酸血症性肾病模型,设立空白组、模型组、苍术白虎汤高、中、低剂量组(6、3、1. 5g·kg^(-1))、苯溴马隆组(20mg·kg^(-1)),连续给药14天。于实验结束后将各组小鼠眼球采血,分光光度法检测其血清中尿酸(BUA)、肌酐(CR)及血浆中尿素氮(BUN)水平,测定血清中黄嘌呤氧化酶(XOD)活性。取双侧肾脏组织匀浆,ELISA法检测肾脏组织中IL-1β、TGF-β1含量。结果:苍术白虎汤可显著降低血清BUA、CR及血浆BUN含量,抑制血清XOD活性及肾脏组织中IL-1β、TGF-β1的表达。结论:苍术白虎汤可通过抑制XOD活性而减少BUA的生成,同时抑制肾脏组织中IL-1β、TGF-β1的表达而发挥对肾脏的保护作用,从而降低BUN及CR的含量。此为苍术白虎汤对高尿酸血症性肾病模型可能的作用机制。Objective:To observe the curative effect of Cangzhu Baihu Decoction for hyperuricemic nephropathy mice and explore the mechanism.Methods:Animal model was made by Adenine(100mg·kg-1)and Ethylamine butanol(250mg·kg-1).The animals were divided into normalgroup,modelgroup,positive drug Benzbromarone(20mg·kg-1),the low dose of Cangzhu Baihu Decoctiongroup(1.5g·kg-1),the middle dose(3g·kg-1)and high dose(6g·kg-1).Then,the drug with different doses wasgiven the medicine,continuously 14 days.At the end of the experiment,the eyeball blood and kidney was collected from eachgroup.Then,the serum level of UA,BUN,CR,XOD and the IL-1β,TGF-β1 in kidney tissue were measured.Results:Compared to the modelgroup,serum BUA,CR,BUN and XOD levels of the Cangzhu Baihu Decoctiongroup was significantly reduced,and downregulated IL-1β,TGF-β1 expression.Conclusion:Cangzhu Baihu Decoctiongroup can reduce BUA production by supressing the activity of XOD.In the meanwhile,Cangzhu Baihu Decoctiongroup can play the protection of the kidney by inhibiting the expressions of IL-1βand TGF-β1 and reduce the serum level of BUN and CR.This is a possible mechanism for the treatment of hyperuricemia nephropathy model by Cangzhu Baihu Decoctiongroup.

关 键 词：高尿酸血症性肾病 苍术白虎汤 白细胞介素1Β 转化生长因子Β1 

分 类 号：R285.5[医药卫生—中药学]