延龄草总皂苷AB-8大孔树脂纯化工艺研究  

Study on Purification of Total Saponins in Trillium tschonoskii with AB-8 Mmacroporous Resin

作　　者：贺丽莹 王晶 肖萌 赵凡[1,2] 闫琦 赵晖 李春[3] 邹海艳 HE Li-ying;WANG Jing;XIAO Meng;ZHAO Fan;YAN Qi;ZHAO Hui;LI Chun;ZOU Haiyan(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China;Beijing Key Lab of TCM Collateral Disease Theory Research,Beijing 100069,China;Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]首都医科大学中医药学院,北京100069 [2]中医络病研究北京市重点实验室,北京100069 [3]中国中医科学院中药研究所,北京100700

出　　处：《时珍国医国药》2018年第12期2870-2872,共3页Lishizhen Medicine and Materia Medica Research

基　　金：首都医科大学本科生第二课堂(D2KT2018129);科研创新项目(XSKY2018117);北京高等学校高水平人才交叉培养"实培计划"项目;北京市教育委员会科技发展计划面上项目(KM201510025011)

摘　　要：目的研究AB-8大孔树脂纯化延龄草总皂苷的工艺。方法以总皂苷和主要皂苷成分{重楼皂苷VII、VI和偏诺皂苷元-3-O-α-L-鼠李糖基(1→4)-[O-α-L-鼠李糖基(1→2)]-O-β-D-葡萄糖苷(PRRG)}的含量为指标,考察延龄草提取物大孔树脂分离的参数,初步确定延龄草总皂苷的制备工艺。结果 AB-8大孔树脂制备延龄草总皂苷的工艺参数为:上样浓度为生药0. 05g/m L,上样量为6倍柱床体积(BV),洗脱流速0. 5mL/min,用蒸馏水(5BV)、20%乙醇(3BV)依次洗脱除去杂质,70%乙醇洗脱(7BV)制备总皂苷,总皂苷的转移率可达87. 30%。结论 AB-8大孔树脂能有效富集和纯化延龄草总皂苷,所确定的工艺条件为该药物的开发利用提供了依据。Objective To investigate the purification methods of total saponins in Trillium tschonoskii( TSTT) with AB-8 macroporous resin. Methods The dynamic adsorption and desorption experiments have been carried out on a AB-8 resin packed column to optimize the separation process of TSTT from the crude extracts,using the contents of total saponin and the three main compounds( paris saponin VI,VII and pennogenin-3-O-α-L-rhamnopyranosyl( 1→4)-[O-α-L-rhamnopyranosyl( 1→2)〗-O-β-D-glucopyranoside( PRRG)) as indicators. Results The optimum parameters for purification of TSTT by AB-8 resin were as follows:( 1) The concentration of crude drug in a sample solution was 0. 05 g/m L with a processing volume of 6 bed volume( BV),and the flow rate was 0. 5 m L/min;( 2) For desorption,the column was eluented with water( 5 BV),20% ethanol-water( 3 BV) and 70% ethanol-water( 6 BV) successively,and the total saponin were enriched in the 70%ethanol elution with high transfer rate( 87. 30%). Conclusion The preparative separation of TSTT can be easily and efficiently achieved by using AB-8 macroporous resin,the method developed will provide a potential approach for its wide pharmaceutical use.

关 键 词：延龄草 总皂苷 AB-8大孔树脂 分离纯化 

分 类 号：R284.2[医药卫生—中药学]