化痰活血扶正方及其拆方药组对模型大鼠VEGF及TGF-β1的影响  

Influence of VEGF and TGF-1 on the Liver Fibrosis in Model Rats after Reducing Phlegm and Activating Blood Fuzheng Decoction and its Decomposed Formula Groups

作　　者：唐杨[1] 郑保平[1] 林唐唐[1] 刘海华[1] 肖海[1] 韩立民[1] TANG Yang;ZHENG Bao-ping;LIN Tang-tang;LIU Hai-hua;XIAO Hai;HAN Li-min(First Affiliated Hospital of Garman Medical University,Ganzhou,Jiangxi,341000,China)

机构地区：[1]赣南医学院第一附属医院,江西赣州341000

出　　处：《时珍国医国药》2018年第12期2921-2925,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81460707)

摘　　要：目的观察化痰活血扶正方拆方药组对肝纤维化大鼠模型血管内皮细胞生长因子(VEGF)及转化生长因子(TGF-β1)表达的影响,并探讨其作用机制。方法选择雄性SD大鼠(清洁级) 90只,随机分为空白对照组(K6只)、模型组(M6只)、秋水仙碱组(Q6只)、低中高剂量活血化瘀组(XD、XZ、XG各6只)、低中高剂量扶正组(FD、FZ、FG各6只)、低中高剂量化痰组(TD、TZ、TG各6只)、低中高剂量化痰活血扶正组(QD、QZ、QG各6只)。除K组未予造模处理外,其余各组大鼠均予10%CCl4橄榄油溶液按3ml/kg剂量皮下注射,每周2次,共8周,复制大鼠肝纤维化模型。除空白对照组未予以灌胃处理外,模型组在造模同时予以大鼠专用无菌水,灌胃体积1ml/100g;其余各组在造模同时即经口灌胃给予相应药液,灌胃体积1ml/100g,秋水仙碱按常用量配制予以灌胃,低、中、高剂量分别为常用量的5倍、10倍、15倍而配制相应浓度药物予以灌胃。8周时间点,分别于模型组、活血药低中高剂量组、扶正药低中高剂量组、化痰药低中高剂量组、化痰活血扶正药低中高剂量组处死所有大鼠,取肝组织。所取肝组织送于PCR检测,待数据结果出来后予以统计分析,观察治疗前后各组间的VEGFmRNA及TGF-β1mRNA的表达情况。结果使用化痰活血扶正方拆方各药组干预后,与对照组相比,模型组的VEGFmRNA及TGF-β1mRNA的表达显著升高(P <0. 05),差异具有统计学意义;各组用药后,Q组、XD组、XZ组、XG组、FD组、FZ组、FG组、TD组、TZ组、TG组、QD组、QZ组、QG组组的VEGFmRNA表达与模型组相比均有显著下降(P <0. 05),差异具有统计学意义; XZ组、XG组、FZ组、FG组、TD组及QG组的TGF-β1mRNA表达与模型组相比均有显著下降(P <0. 05),差异具有统计学意义。结论化痰活血扶正方拆方药组对四氯化碳诱导的肝纤维化实验大鼠肝组织中的VEGFmRNA及TGF-β1mRNA的表达有降低作用,因此考虑该�Objective To observe the effect of VEGF and TGF-1 on the Liver Fibrosis in Model Rats after reducing phlegm and activating blood Fuzheng decoction and its decomposed formulas,and to explore its mechanism. Methods 90 male SD rats( clean) were randomly divided into blank control group( K6 rats),model group( M6 rats),colchicine group( Q6 rats),low,middle and high doses of activating blood to remove blood stasis group( XD,XZ,XG 6 rats),low,middle and high dose of Fuzheng group( FD,FZ,FG 6 rats),low,middle and high dose of reducing phlegm group( TD,TZ,TG 6 rats),low,middle and high dose of reducing phlegm and activating group( QD,QZ,QG6 rats). In addition to the normal control group hadn’t been builting model,the rest of the group rats were given 10 % CCL4 olive oil solution according to the3 ml/kg dose of subcutaneous injection,twice a week,a total of 12 weeks,copy the model of rat liver fibrosis. In addition to the normal control group,no gavage treatment was given,the model group was also given sterile water for rats at the same time of model making,and the volume of gavage was 1 ml/100 g. Groups in the model at the same time were filled the stomach through the mouth with corresponding solution,gastric volume 1 ml/100 g,dosage of colchicine by often made up to fill the stomach,low,medium and high doses respectively often choosen five times,ten times,15 times that made up the corresponding concentration drug lavage. 12 w time points,respectively in the model group,activating blood of low and middle dose group,Fuzheng low and middle dose group,reducing phlegm low and middle dose group,reducing phlegm and activating blood Fuzheng to be put to death all the rats in the lower middle and high dose groups,got the liver tissue. Liver tissue was sent to PCR for detection,and statistical analysis was carried out after the data results were obtained. In this paper,VEGFmRNA and TGF-1 mRNA expression in each group before and after treatment were observed. Results Compared with the control group,the expression of VEGFmRNA and

关 键 词：肝纤维化 化痰法 化痰活血扶正方 拆方 

分 类 号：R285.5[医药卫生—中药学]