三苯胺类荧光探针的合成与细胞成像  

Synthesis and Cell Imaging of Triphenylamine-based Fluorescent Probe

作　　者：尹培 马拉毛草 马恒昌[1] YIN Pei;MALA Mao-cao;MA Heng-chang(Key Laboratory of Eco-Environment-Related Polymer Materials of Ministry of Education,Key Laboratory ofPolymer Materials of Gansu Province.College of Chemistry and Chemical Engineering,Northwest Normal University,Lanzhou 730070,Gansu,China;Shaw Library,Northwest Normal University,Lanzhou 730070,Gansu,China)

机构地区：[1]西北师范大学化学化工学院甘肃省高分子材料重点实验室生态环境相关高分子材料教育部重点实验室,甘肃兰州730070 [2]西北师范大学逸夫图书馆,甘肃兰州730070

出　　处：《精细化工》2019年第2期212-217,共6页Fine Chemicals

基　　金：国家自然科学基金(21764012)~~

摘　　要：以三苯胺为原料,通过Vilsmeier-Haack和McMurry反应合成了一个碳碳双键连接两个三苯胺的荧光分子1,2-二(三苯胺基)乙烯(TPAS)。采用紫外-可见光谱和荧光光谱对TPAS的光学性质进行了考察。结果表明:TPAS有很强的荧光发射性能,荧光发射为蓝光,荧光强度与浓度的线性关系较好,且溶剂化效应不强,光稳定性良好。采用扫描电镜测试了TPAS的聚集态行为。结果显示:当水的体积分数增大后,TPAS分子聚集,形貌改变。采用MTT法对TPAS进行了细胞毒性测试。结果表明,不同TPAS浓度下,细胞的存活度都在80%以上,说明TPAS对细胞的毒性很低,生物相容性好。并实现了TPAS对A549的活细胞成像。4,4’-(Ethene-1,2-diyl)bis(N,N-diphenylaniline)(TPAS), a compound was characteristic of carbon-carbon double bond connecting triphenylamines was synthesized via Vilsmeier-Haack reaction and Mc Murry reaction using triphenylamine as a raw material. The optical properties of TPAS were studied by UV-vis absorption spectrum and fluorescence spectrum. The results showed that TPAS had strong fluorescence emission, and the fluorescence emission was blue light. The fluorescence intensity had a good linear relationship with the TPAS concentration. The solvation effect was not strong, and the optical stability was good. The aggregation of TPAS was characterized by scanning electron microscope(SEM). The results revealed that when the volume fraction of water increased, the TPAS molecules accumulated and the morphology changed. The cytotoxicity of TPAS was tested by MTT assays, the cell viability was above 80% at different concentrations of TPAS, indicating that TPAS had low toxicity to cells and good biocompatibility. In addition, the imaging was successfully achieved in living human lung cancer A549 cells.

关 键 词：三苯胺 荧光探针 光学性质 生物相容性 细胞成像 功能材料 

分 类 号：O621.2[理学—有机化学]