一株低温秸秆纤维素降解菌的分离、鉴定及降解特性  

Isolation, Identification and Characteristics Analysis of a Straw-cellulose Degradation Strain adaptable for Low-temperature

作　　者：李娜[1] 韩永武[2] 金勋[1] 王丽娜[1] 潘红丽[1] 芮海英[1] 于吉东[1] 杨柳[1] 韩冰[1] 齐国超[1] LI Na;HAN Yong-wu;JIN Xun;WANG Li-na;PAN Hong-li;RUI Hai-ying;YU Ji-dong;YANG Liu;HAN Bing;QI Guo-chao(Daqing Branch of Heilongjiang Academy of Agricultural Sciences,Daqing 163316;Keshan Branch of Heilongjiang Academy of Agricultural Sciences,Keshan 161600,China)

机构地区：[1]黑龙江省农业科学院大庆分院,黑龙江大庆163316 [2]黑龙江省农业科学院克山分院,黑龙江克山161600

出　　处：《玉米科学》2019年第1期159-163,共5页Journal of Maize Sciences

基　　金：黑龙江省农业科技创新工程(QN023);大庆市指导性科技计划项目(zd-2016-145)

摘　　要：本研究在18℃条件下,利用赫奇逊滤纸培养基分离纯化菌株,根据透明圈和滤纸降解情况筛选菌株,最终通过酶活分析和秸秆降解率确定目的菌株,通过形态学观察与ITS基因序列分析对目的菌株进行初步鉴定,分离筛选高效低温秸秆纤维素降解菌并研究其产酶特性。结果表明,从小兴安岭山区土壤中分离到1株在低温下具有较强纤维素降解能力的真菌菌株C1,15 d内对秸秆的降解能力达55.6%,滤纸酶活和CMC酶活分别为18.4 U/mL和54.3 U/mL,初步鉴定菌株C1为青霉菌属(Penicillium sp.),具有进一步研究开发的潜力。This study aimed to isolate and screen straw-cellulose degradation strain adapted for low-tempera-ture.At 18℃,we isolated and purified many strains by Hutchinson medium,further screened them according to the transparent circles and filter paper degradation,and then determined the target one through enzyme activity analysis and straw-cellulose degradation rate.The selected strain was preliminarily identified by its morphological character-istics,and then preformed internal transcribed spacers(ITS)gene sequence analysis.Finally,we successfully ob-tained a fungus from Xiaoxing’an Mountains collections and named it as C1,which was highly efficiency for cellu-lose degradation at low temperature,with straw-cellulose degradation rate of 55.6%within 15 d,and filter paper and CMC enzyme activities being 18.4 and 54.3 U/mL,respectively.Strain C1 was identified as a kind of Penicillium sp.,which has the potential of further research.

关 键 词：玉米 秸秆 纤维素降解菌 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]