菖蒲郁金汤化裁方对2型糖尿病合并非酒精性脂肪肝大鼠SREBP-1c表达和胰岛素抵抗的影响  

Effect of Changpu Yujin Decoction on insulin resistance and expression of SREBP-1c of rats with type 2 diabetes mellitus(T2DM)complicated with nonalcoholic fatty liver disease

作　　者：张哲滔[1] 车念聪[1] 郑亚琳[1] 田甜[1] 魏晓艺 刘晔[1] ZHANG Zhe-tao;CHE Nian-cong;ZHENG Ya-lin;TIAN Tian;WEI Xiao-yi;LIU Ye(College of Traditional Chinese Medicine, Capital Medical University, Beijing Key Lab for TCM Collateral Research, Beijing 100069,China)

机构地区：[1]首都医科大学中医药学院中医络病研究北京市重点实验室,北京100069

出　　处：《北京中医药》2019年第1期17-20,F0003,共5页Beijing Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81603564);北京市教育委员会科技计划一般项目(KM201710025013)

摘　　要：目的观察菖蒲郁金汤化裁方对2型糖尿病(T2DM)合并非酒精性脂肪肝(NAFLD)大鼠肝脏组织病理、胰岛素抵抗(IR)和固醇调节元件结合蛋白-1c(SREBP-1c)表达的影响。方法将32只SD大鼠分成正常组8只和模型组24只,采用高脂饮食结合链脲佐菌素(STZ)建立T2DM合并NAFLD大鼠模型。造模成功后,再将成模大鼠随机分成中药组、阳性药组、模型组各8只。中药组灌服菖蒲郁金汤化裁方药液,阳性药组灌服二甲双胍粉末混悬液,正常组和模型组给予等体积生理盐水灌胃,8周后麻醉处死取材。HE染色观察各组大鼠肝脏病理变化,检测各组大鼠空腹血糖(FBG)、胰岛素(INS)含量,并计算胰岛素抵抗指数(HOMA-IR),RT-PCR法测定大鼠肝脏SREBP-1c的表达水平。结果与模型组相比,中药组肝脏脂肪变现象明显改善,INS和HOMA-IR水平明显降低(P<0.05或P<0.01),SREBP-1c在肝脏内的表达水平明显降低。结论菖蒲郁金汤化裁方对T2DM合并NAFLD大鼠有较明显的治疗作用,其作用机制可能与降低SREBP-1c的表达水平,从而减少肝脏脂肪沉积和细胞变性,并改善IR有关。Objective To study the effect of Changpu Yujin Decoction on liver tissue pathology,insulin resistance(IR)and expression of liver sterol regulatory element binding protein-1 c(SREBP-1 c)of rats with type 2 diabetes mellitus(T2 DM)complicated with nonalcoholic fatty liver disease(NAFLD). Methods A total of 32 SD rats were randomized into two groups: the control group of 8 and the model group of 24. The model group were induced T2 DM with NAFLD by streptozotocin(STZ)and high-fat diet. After successfully establishing the models,they were randomly divided into Chinese herbal medicine group,Metformin group and model group of 8 each. The Chinese herbal medicine group was given Changpu Yujin Decoction solution. The Metformin group was given the metformin powder suspension. The control group and the model group were given an equal volume of 0.9% sodium chloride aqueous solution. Eight weeks later,the rats were given anesthetic death for sampling. The pathological changes of liver in each group were observed by HE staining. The fasting blood glucose(FBG)and insulin content(INS)of each group were measured and the insulin resistance index(HOMA-IR)calculated. The level of expression of liver SREBP-1 c was determined by RT-PCR. Results Compared with the model group,Changpu Yujin Decoction could significantly alleviate the liver steatosis of T2 DM with NAFLD,reduce the levels of INS and HOMA-IR(P<0.05 or P<0.01),and decrease the the level of SREBP-1 c expression in the liver. Conclusion Changpu Yujin Decoction has a significant therapeutic effect on T2 DM with NAFLD in rats. The mechanism may be related to the reduction of SREBP-1 c expression,thereby reducing liver fat deposition,cell degeneration and alleviate IR.

关 键 词：2型糖尿病 非酒精性脂肪肝 胰岛素抵抗 SREBP-1C 

分 类 号：R285.5[医药卫生—中药学]