高效液相色谱法测定消癌平片中通关藤苷G含量  

Content Determination of Tenacissoside G in Xiao'aiping Tablets by HPLC

作　　者：王祁民 李亚静 安静[1] 孟璐 马银玲[1] WANG Qimin;LI Yajing;AN Jing;MENG Lu;MA Yinling(Department of Pharmacy,Hebei General Hospital,Shijiazhuang,Hebei,China 050051;Graduate School of Hebei Medical University,Shijiazhuang,Hebei,China 050017)

机构地区：[1]河北省人民医院药学部,河北石家庄050051 [2]河北医科大学研究生学院,河北石家庄050017

出　　处：《中国药业》2019年第8期17-19,共3页China Pharmaceuticals

基　　金：河北省中医药管理局2018年度中医药类科研课题计划[冀中医药〔2017〕67号-2018073];河北省2017年度医学科学研究重点课题计划[20170245];河北省2018年度医学科学研究重点课题计划[20180176]

摘　　要：目的建立测定消癌平片中通关藤苷G含量的高效液相色谱法。方法色谱柱为Symmetry C_(18)柱(250 mm×4. 6 mm,5μm),流动相为乙腈-0. 1%磷酸溶液(55∶45,V/V),流速为1 mL/min,检测波长为230 nm,柱温为25℃,进样量为20μL。结果通关藤苷G质量浓度线性范围为2～100μg/mL;加样回收率为97. 50%～99. 00%,RSD为0. 73%(n=9)。结论该方法可靠、简便、准确,可用于消癌平片的质量控制。Objective To establish an HPLC method for the content determination of tenacissoside G in Xiao’aiping Tablets. Methods The chromatographic column was Symmetry C18 column( 250 mm × 4. 6 mm,5 μm),the mobile phase was acetonitrile-0. 1% phosphoric acid( 55 ∶ 45, V/V),the flow rate was 1 mL/min,the detection wavelength was 230 nm,the column temperature was 25 ℃,and the sample size was 20 μL. Results The tenacissoside G showed good relationship in the range of 2-100 μg/mL. The recovery rates ranged from 97. 50% to 99. 00%, and RSD was 0. 73%( n = 9). Conclusion The method is reliable, simple and accurate, which can be used for the quality control of Xiao’aiping Tablets.

关 键 词：高效液相色谱法 消癌平片 通关藤苷G 含量测定 质量控制 

分 类 号：R917[医药卫生—药物分析学] R927.2[医药卫生—药学]