GABAA受体在七氟醚抑制新生大鼠延髓呼吸中枢吸气神经元放电中的作用  

Role of GABAA receptors in sevoflurane-induced inhibition of discharge activities of inspiratory neurons in medullary respiratory center of neonatal rats

作　　者：郑奇辉[1] 刘伟[1] 王书宝[1] 黄生辉[1] 王迎斌[1] Zheng Qihui;Liu Wei;Wang Shubao;Huang Shenghui;Wang Yingbin(Department of Anesthesiology,Lanzhou University Second Hospital,Lanzhou 730030,China)

机构地区：[1]兰州大学第二医院麻醉科,730030

出　　处：《中华麻醉学杂志》2018年第11期1311-1313,共3页Chinese Journal of Anesthesiology

基　　金：甘肃省卫生行业科研计划(GSWSKY-2015-61)。

摘　　要：目的评价GABAA受体在七氟醚影响新生大鼠延髓呼吸中枢吸气神经元放电中的作用。方法制作新生大鼠(0~4d)离体延髓脑片标本15例,主要包含面神经后核内侧区(mNRF区),完整保留舌下神经根,以人工脑脊液灌流脑片,同步记录吸气神经元及舌下神经根呼吸节律性放电活动。待放电活动稳定后,在灌流液中分别单独给予人工脑脊液,给予5%七氟醚,给予GABAA受体阻断剂荷包牡丹碱(10μmol/L),给予5%七氟醚+GABAA受体阻断剂荷包牡丹碱(10μmol/L),记录吸气神经元放电的呼吸周期(RC)、吸气时程(TI)、放电积分幅度(IA)和单位放电频率(PFn)的变化。结果 与单独给予人工脑脊液比较,给予七氟醚后,RC延长,TI缩短,IA减小,PFn减慢(P<0.05);给予荷包牡丹碱后,RC缩短,IA和PFn增加(P<0.05),TI差异无统计学意义(P>0.05);同时给予七氟醚和荷包牡丹碱与单独给予七氟醚比较,RC缩短,TI延长,IA和PFn增加(P<0.05)。结论在新生大鼠延髓呼吸中枢,七氟醚通过GABAA受体抑制吸气神经元放电活动。Objective To evaluate the role of GABAA receptors in sevoflurane-induced inhibition of the discharge activities of inspiratory neurons in the medullary respiratory center of neonatal rats.Methods The medulla oblongata slices of neonatal rats(aged 0-4 days)including the medial region of the nucleus retrofacialis with the hypoglossal nerve rootlets retained were prepared.The slices were perfused with artificial cerebrospinal fluid(ACSF),and the activity of the inspiratory neurons in the medial region of the nucleus retrofacialis and the respiratory rhythmical discharge activity of the hypoglossal nerve rootlets were simultaneously recorded using microelectrodes and suction electrodes,respectively.The ACSF,5% sevoflurane,GABAA receptor blocker bicuculline 10 μmol/L and combination of 5% sevoflurane and 10 μmol/L bicuculline were added to the perfusion liquid after the discharge activity was stable.The respiratory cycle(RC),inspiratory time(TI),integral amplitude(IA)and changes in peak frequency(PFn)of the inspiratory neurons were recorded.Results Compared with that after giving ACSF,RC was significantly prolonged,TI was shortened,and IA and PFn were decreased after giving sevoflurane,and RC was significantly shortened,IA and PFn were increased(P<0.05),and no significant change was found in TI after giving bicuculline(P>0.05).Compared with that after giving sevoflurane,RC was significantly shortened,TI was prolonged,and IA and PFn were increased after giving sevoflurane and bicuculline(P<0.05).Conclusion Sevoflurane inhibits the discharge activities of inspiratory neurons through GABAA receptors in the medullary respiratory center of neonatal rats.

关 键 词：麻醉药 吸入 呼吸中枢 神经元 

分 类 号：R614[医药卫生—麻醉学]